| restriction endonuclease fingerprinting analysis of canadian isolates of aeromonas salmonicida. | restriction endonuclease fingerprinting (ref) analysis was used to examine total cellular dna prepared from 56 independent field isolates of the fish pathogen, aeromonas salmonicida. dna was digested singly with the restriction enzymes ecori and hindiii, and the resulting fragments separated by polyacrylamide gel electrophoresis and visualized by silver staining. the ref patterns of typical isolates of a. salmonicida subsp. salmonicida were distinct from those of a. hydrophila, a. salmonicida su ... | 1990 | 2159364 |
| lipolytic bacteria in the ottawa river. | lipolytic bacteria were isolated from two stations on brewery creek, an arm of the ottawa river, during the winter of 1971-72. total counts were approximately sevenfold higher at the more polluted downstream station, whereas lipolytic counts were about 100-fold higher. at this station, significantly more lipolytic bacteria grew on plates incubated at 20 c than at 4 c, suggesting that the population was comprised of both mesophiles and psychrophiles. however, at the upstream station, approximatel ... | 1973 | 4762394 |
| hatchery-level predictive values for infectious pancreatic necrosis virus and aeromonas salmonicida in ontario, canada. | the probability and uncertainty of correctly classifying the ipnv and aeromonas salmonicida status of fish-rearing and natural sites in ontario were estimated through monte carlo simulations. propagating several uncertain inputs showed the extent to which natural variability and our present lack of knowledge affect the probability of site misclassification. for the scenarios investigated, the site-level negative predictive values (snpvs) were high and fairly constant. the site-level positive pre ... | 2001 | 11154785 |
| host factors associated with the detection of aeromonas salmonicida and yersinia ruckeri in ontario, canada government fish hatcheries. | this paper presents an epidemiological investigation of ontario ministry of natural resources fish health laboratory data from 1981 to 1997, to determine whether fish species and age were associated with lot-level detection of aeromonas salmonicida and yersinia ruckeri in hatchery fish. in stepwise logistic regression, the species brook trout and back-cross (lake trout crossed with the hybrid "splake") were more likely to test a. salmonicida-positive compared to all other species reared in the h ... | 2001 | 11311951 |
| simulating the effect of eliminating routine bacterial screening on the negative predictive value of the ontario fish hatchery disease monitoring program. | we determined the impact of eliminating routine screening for aeromonas salmonicida and yersinia ruckeri on the efficacy of the ontario ministry of natural resources (omnr) fish disease monitoring program, using monte carlo simulation. because the main purpose of the program is to prevent transferring infected fish among omnr hatcheries, or to wild fish populations through stocking waterways, the hatchery-level negative predictive value (hnpv) was used as an indicator of monitoring efficacy. the ... | 2001 | 11448498 |
| isolation of aeromonas salmonicida from sea lamprey (petromyzon marinus) with furuncle-like lesions in lake ontario. | for the past six decades, parasitic sea lampreys (petromyzon marinus) have caused devastating losses to salmonid fisheries in the great lakes. to reduce the number of sea lampreys, the great lakes fishery commission began a large-scale program based on trapping male sea lampreys, sterilizing them, and releasing sterile males back into streams to compete with fertile males for spawning females. the transfer of lampreys among lakes can potentially lead to the transfer of various pathogens, and thi ... | 2007 | 17984256 |
| dna-based real-time detection and quantification of aeromonads from fresh water beaches on lake ontario. | the present study was designed to develop a novel, rapid, direct dna-based protocol to enumerate aeromonads in recreational waters. an aeromonas genus-specific real-time quantitative polymerase chain reaction (q-pcr) protocol was developed and optimized using newly designed genus-specific oligonucleotide primers derived from the gyrase b subunit (gyrb) gene. a standard curve was developed based on the pcr protocol with a minimum quantification limit of 10 cell equivalents ml(-1) achieved using a ... | 2009 | 19240357 |