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serological and clinical surveillance studies to validate reported foot-and-mouth disease free status in tsirang district of bhutan.serological and clinical studies were conducted between march 2009 and august 2010 to validate the foot-and-mouth disease free status of tsirang district of bhutan as determined by the country's passive surveillance system. randomised (first survey) and targeted (third survey) samplings, with subsequent follow-up samplings (second and fourth), were conducted on fmd-susceptible animals to detect the disease at a design prevalence of 25% and 20% at the individual animal-level and village-level, re ...201122172605
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