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study of lyssaviruses of bat origin as a source of rabies for other animal species in the state of rio de janeiro, brazil.an epidemic-geographic rabies study was carried out in which 72 animal and human brain samples were analyzed for lyssaviruses by a direct immunofluorescent technique (dift) and a reverse transcriptase-polymerase chain reaction (rt-pcr) assay. fifty-two samples were also tested by a mouse inoculation test. lyssavirus rna was detected in 60 of 72 samples. five dift-negative bat samples tested by a nested pcr assay showed evidence of the presence of rabies virus rna. sequencing of amplified rabies ...200312932103
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