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sterolibacterium denitrificans gen. nov., sp. nov., a novel cholesterol-oxidizing, denitrifying member of the beta-proteobacteria.a bacterial strain (chol-1s(t)) that is able to oxidize cholesterol to co2 and reduce nitrate to dinitrogen was enriched and isolated from an upflow sludge bed (usb) anoxic reactor that treats sanitary landfill leachate from the city of montevideo, uruguay. cells of strain chol-1s(t) were gram-negative, rod-shaped to slightly curved, measured 0.5-0.6 x 1.0-1.3 microm and were motile by a single polar flagellum. strain chol-1s(t) grew optimally at 30-32 degrees c and ph 7.0, with a doubling time ...200312892131
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