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454 pyrosequencing reveals bacterial diversity of activated sludge from 14 sewage treatment plants.activated sludge (as) contains highly complex microbial communities. in this study, pcr-based 454 pyrosequencing was applied to investigate the bacterial communities of as samples from 14 sewage treatment plants of asia (mainland china, hong kong, and singapore), and north america (canada and the united states). a total of 259 k effective sequences of 16s rrna gene v4 region were obtained from these as samples. these sequences revealed huge amount of operational taxonomic units (otus) in as, tha ...201122170428
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