Publications

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evolutionary consequences of g9 rotaviruses circulating in thailand.infection with group a rotaviruses is the main cause of acute gastroenteritis in infants and young children worldwide. rotavirus g9 is recognized as the fifth most predominant g genotype that spreads throughout the world. in this study, we describe the changing distribution of rotavirus g9 genotype in chiang mai, thailand, from 1989 to 2005. molecular genetic evolutionary analysis of the g9 rotavirus vp7 gene was performed. the g9 rotavirus in thailand was first detected in chiang mai in 1989 wi ...200919716437
genotypic linkages of vp4, vp6, vp7, nsp4, nsp5 genes of rotaviruses circulating among children with acute gastroenteritis in thailand.rotavirus is the main cause of acute viral gastroenteritis in infants and young children worldwide. surveillance of group a rotavirus has been conducted in chiang mai, thailand since 1987 up to 2004 and those studies revealed that group a rotavirus was responsible for about 20-61% of diarrheal diseases in hospitalized cases. in this study, we reported the continuing surveillance of group a rotavirus in 2005 and found that group a rotavirus was detected in 43 out of 147 (29.3%) stool samples. fiv ...201020223298
changing pattern of rotavirus g genotype distribution in chiang mai, thailand from 2002 to 2004: decline of g9 and reemergence of g1 and g2.group a rotaviruses are the most common cause of acute viral diarrhea in humans and animals throughout the world. previous surveillance studies of group a rotaviruses in thailand indicated that the dominant types of rotaviruses were changing from time to time. during 2000 and 2001, the g9 rotavirus emerged as the most prevalent genotype, with an exceptionally high frequency (91.6%) in chiang mai, thailand. in the year 2002-2004, group a rotavirus was detected in 98 out of 263 (37.3%) fecal speci ...200717854032
molecular characterization of rare g3p[9] rotavirus strains isolated from children hospitalized with acute gastroenteritis.in 2004, an epidemiological survey of human rotavirus infection in chiang mai, thailand detected two uncommon human rotavirus strains (cmh120/04 and cmh134/04) bearing au-1-like g3p[9] genotypes in 1 year old children hospitalized with acute gastroenteritis. the cmh120/04 and cmh134/04 rotavirus strains were characterized by molecular analyses of their vp6, vp7, vp8*, and nsp4 gene segments as well as the determination of rna patterns by polyacrylamide gel electrophoresis (page). analysis of the ...200717457907
multiple combinations of p[13]-like genotype with g3, g4, and g5 in porcine rotaviruses.epidemiological surveillance of porcine rotavirus (porv) strains was carried out in chiang mai province, thailand, from 2002 to 2003, and eight rotavirus isolates could not be completely typed by pcr. of these, six were g3 and one was g4 and displayed a p-nontypeable genotype, while another isolate was both g and p nontypeable. analysis of a partial vp4 gene of all eight p-nontypeable strains revealed a high degree of amino acid sequence identities (94.7% to 100%), suggesting that they belonged ...200818234859
molecular characterization of vp4, vp6, vp7, nsp4, and nsp5/6 genes identifies an unusual g3p[10] human rotavirus strain.an unusual strain of human rotavirus g3p[10] (cmh079/05) was detected in a stool sample of a 2-year-old child admitted to the hospital with severe diarrhea in chiang mai, thailand. analysis of the vp7 gene sequence revealed highest identities with unusual human rotavirus g3 strain cmh222 at 98.7% on the nucleotide and 99.6% on the amino acid levels. phylogenetic analysis of the vp7 sequence confirmed that the cmh079/05 strain formed a cluster with g3 rotavirus reference strains and showed the cl ...200919031442
emergence of human g9 rotavirus with an exceptionally high frequency in children admitted to hospital with diarrhea in chiang mai, thailand.among 315 fecal specimens collected from children hospitalized with diarrhea in chiang mai, thailand, in 2000-2001, group a rotavirus was detected in 107 (34.0%). of these, 98 (91.6%) were g9, 6 (5.6%) were g3 and 3 (2.8%) were g2, respectively. identification of their p-types demonstrated that 103 (96.3%) were p[8], 3 (2.8%) were p[4], and 1 (0.9%) was p[3] genotypes. determination of g- and p-type combination revealed that all of g9 isolates were associated with p[8]. g9p[8] was the most predo ...200616372282
antigenic and genetic analyses of human rotaviruses in chiang mai, thailand: evidence for a close relationship between human and animal rotaviruses.serotyping of group a rotaviruses obtained from stools of 158 diarrheic patients in chiang mai, thailand, by elisa with monoclonal antibodies revealed a yearly change in the prevalence of individual serotypes. three unusual human rotavirus strains were isolated. strain mc35 had subgroup i-serotype 10 antigen and a long rna electrophoretic type, a property hitherto found only in bovine rotaviruses. rna-rna hybridization tests showed that the strain is more closely related genetically to bovine th ...19921378870
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