| mutations in the gyra subunit of dna gyrase and the parc subunit of topoisomerase iv in clinical strains of fluoroquinolone-resistant shigella in anhui, china. | in this research 26 shigella isolates were examined by pcr and direct nucleotide sequencing for genetic alterations in the quinolone-resistance determining regions (qrdrs). we tested for the presence of qnr genes by pcr in 91 strains, but no qnr genes were found. the results did show, however, some novel mutations at codon 83 of gyra (ser-->ile) and codon 64 of parc (ala64-->cys, ala64-->asp), which were related to fluroquinolone resistance. | 2007 | 17483803 |
| prevalence of plasmid-mediated quinolone-resistance determinants in shigella flexneri isolates from anhui province, china. | quinolones are used extensively to treat shigella flexneri infection, and plasmid-mediated quinolone resistance (pmqr) determinants were recently reported. a total of 26 s. flexneri isolates collected from anhui province, china, in 2005 were screened for pmqr determinants, bla gene, gyra and parc genes, by pcr and sequencing. pmqr determinants were present in 53.8% (14 of 26) of isolates and qnra(1), qnrs(1), qnrs(2), aac(6')-ib-cr and qepa were present in 30.8, 11.5, 3.8, 19.2 and 11.5% of thos ... | 2010 | 20203702 |