| molecular microbial diversity of an agricultural soil in wisconsin. | a culture-independent survey of the soil microbial diversity in a clover-grass pasture in southern wisconsin was conducted by sequence analysis of a universal clone library of genes coding for small-subunit rrna (rdna). a rapid and efficient method for extraction of dna from soils which resulted in highly purified dna with minimal shearing was developed. universal small-subunit-rrna primers were used to amplify dna extracted from the pasture soil. the pcr products were cloned into pgem-t, and ei ... | 1996 | 8787391 |
| sulfate-reducing bacteria-dominated biofilms that precipitate zns in a subsurface circumneutral-ph mine drainage system. | the microbial diversity of zns-forming biofilms in 8 degrees c, circumneutral-ph groundwater in tunnels within the abandoned piquette zn, pb mine (tennyson, wisconsin, usa) has been investigated by molecular methods, fluorescence in situ hybridization (fish), and cultivation techniques. these biofilms are growing on old mine timbers that generate locally anaerobic zones within the mine drainage system. sulfate-reducing bacteria (srb) exclusively of the family desulfobacteriaceae comprise a signi ... | 2004 | 14994175 |
| microbial communities and fecal indicator bacteria associated with cladophora mats on beach sites along lake michigan shores. | a high biomasses of cladophora, a filamentous green alga, is found mainly during the summer along the shores of lake michigan. in this study, the abundance and persistence of the fecal indicator bacterium escherichia coli and sulfate-reducing bacteria (srb) on cladophora mats collected at lake michigan beaches were evaluated using both culture-based and molecular analyses. additionally, 16s rrna gene cloning and sequencing were used to examine the bacterial community composition. overall, e. col ... | 2006 | 16517640 |