development of rt-semi-nested pcr for detection of hepatitis a virus in stool in epidemic conditions. | the purpose of this study was to determine the efficiency of semi-nested pcr in detecting hepatitis a virus (hav) rna. during a 2-year period (1990-1991), hav rna was searched for in shellfish from the french brittany coasts using crna and vrna probes. in january 1992, at the time of a hepatitis a outbreak, 28 stool samples were collected from infected patients (18 adults, 10 children) with anti-hav igm. four samples from subjects with negative hav serology were used as negative controls. nuclei ... | 1994 | 7935509 |