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concordance of clinical and environmental isolates of cryptococcus neoformans var. gattii by random amplification of polymorphic dna analysis and pcr fingerprinting.sixty-one clinical and forty-nine environmental isolates of cryptococcus neoformans var. gattii from australia and the united states were analyzed by random amplification of polymorphic dna (rapd), using 12- to 22-mer primers in pairs, and/or pcr fingerprinting with a single primer derived from the microsatellite core sequence of the wild-type phage m13 (5' gagggtggcggttct 3'). three major genetic profiles were identified by both typing techniques. a single rapd profile (vgi) predominated among ...19968727912
natural environmental sources of cryptococcus neoformans var. gattii.we sought evidence for new environmental sources of cryptococcus neoformans var. gattii by random amplification of polymorphic dna (rapd) analysis of isolates from 29 animals with a restricted territorial range in five australian states. twenty-three of the 29 isolates and 45 of 45 eucalypt isolates tested previously exhibited one rapd profile, vgi. rapd profile vgii was identified in 6 of 17 isolates from domesticated species but in none of 12 native species. four vgii isolates originated from ...19968727913
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