Publications

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prokaryotic expression and potential application of the truncated pcv-2 capsid protein.three pairs of specific primers were designed to amplify the f2-1, f2-2 and xf2-2 truncated sequences of orf2 which encodes the capsid protein of porcine circovirus type 2 (pcv-2). the f2-1 sequence had most of the nls region of orf2, but the f2-2 and xf2-2 genes had the nls region deleted. truncated genes were subcloned into pet-32a(+) vectors to construct recombinant fusion expression vectors. the vectors were then transformed into rosetta(de3) e. coli and expressed by induction of iptg. expre ...201020960305
an investigation of duck circovirus and co-infection in cherry valley ducks in shandong province, china.the co-infection of duck circovirus (ducv) with riemerella anatipestifer (ra) or/and escherichia coli (e. coli) or/and duck hepatitis virus i (dhv-i) in cherry valley ducks in china's shandong province was investigated by using polymerase-chain-reaction (pcr)-based methods. for this study, 742 ducks sampled at random from 70 duck farms during 2006-2007 were examined using pcr and dot-blot hybridisation (dbh) tests. overall the ducv infection rate was 33.29%. compared with those at 2 weeks of age ...200918760549
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