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molecular survey of dirofilaria immitis and dirofilaria repens by new real-time taqman(®) pcr assay in dogs and mosquitoes (diptera: culicidae) in corsica (france).dirofilaria immitis and d. repens are filarioid nematodes of animals and humans, transmitted by the bite of infected mosquitoes. domestic and wild canids are a major natural host and reservoir for these parasites. in this study, we designed a duplex real-time pcr protocol targeting the mitochondrial cytochrome c oxidase subunit i (coi) gene, detecting both d. immitis and d. repens using two primer pairs and two dirofilaria-specific hydrolysable probes. the sensitivity and specificity of the prim ...201728215858
occurrence of dirofilaria immitis and tick-borne infections caused by anaplasma phagocytophilum, borrelia burgdorferi sensu lato and ehrlichia canis in domestic dogs in france: results of a countrywide serologic survey.the occurrence of dirofilaria immitis antigen and antibodies against tick-borne pathogens in french dogs has been analysed based on 1,050 blood samples. serum samples of 919 dogs (group a) were sent for a variety of diagnostic investigations, further 131 dogs (group b) were tested for a tentative diagnosis of heartworm disease. all samples were tested for d. immitis antigen. samples in group a were also tested for specific antibodies against three tick-borne pathogens (anaplasma phagocytophilum, ...200919575231
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