Publications

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prevalence of selected vector-borne organisms and identification of bartonella species dna in north american river otters (lontra canadensis).trapper-killed north american river otters (lontra canadensis) in north carolina, usa, were screened for multiple vector-borne bacteria known to be pathogenic to mammals. blood was collected from 30 carcasses in 2006, from 35 in 2007, and from one live otter in 2008. samples were screened using conventional polymerase chain reaction (pcr) tests for dna from bartonella spp., ehrlichia spp., and spotted fever group rickettsia spp. all samples were negative for rickettsia spp. twelve of 30 samples ...201020688703
bacterial pathogens in ixodid ticks from a piedmont county in north carolina: prevalence of rickettsial organisms.in north carolina, reported human cases of tick-borne illness, specifically rocky mountain spotted fever, have escalated over the past decade. to determine the relative abundance of vectors and to estimate the risk of acquiring a tick-borne illness in peri-residential landscapes, ticks were collected in chatham county, a typical piedmont county and, samples of the ticks were tested for infection with selected bacterial pathogens using real-time quantitative polymerase chain reaction assays. tick ...201020455778
rocky mountain spotted fever vaccine: a regional need.because the killed rickettsia vaccines have failed to provide protection against rocky mountain spotted fever, we approached the problem of protective immunity to rickettsia rickettsii with a closely related, live, less pathogenic spotted fever rickettsia. guinea pigs vaccinated with r conorii produced antibodies to spotted fever group rickettsiae and did not have fever or other signs of illness after challenge with r rickettsii. a vaccine against rocky mountain spotted fever would be useful in ...19846424239
tick-borne diseases in north carolina: is "rickettsia amblyommii" a possible cause of rickettsiosis reported as rocky mountain spotted fever?cases of rocky mountain spotted fever (rmsf) in north carolina have escalated markedly since 2000. in 2005, we identified a county in the piedmont region with high case numbers of rmsf. we collected ticks and examined them for bacterial pathogens using molecular methods to determine if a novel tick vector or spotted fever group rickettsiae (sfgr) might be emerging. amblyomma americanum, the lone star tick, comprised 99.6% of 6,502 specimens collected in suburban landscapes. in contrast, dermacen ...200818447622
the incidence of ehrlichial and rickettsial infection in patients with unexplained fever and recent history of tick bite in central north carolina.we examined the clinical and laboratory findings of a consecutive series of patients from central north carolina presenting with fever and a history of tick bite within the preceding 14 days. evidence of a tick-transmitted pathogen was detected in 16 of 35 patients enrolled over a 2-year period. nine patients were infected with ehrlichia chaffeensis, and 6 were infected with a spotted fever group rickettsia; 1 patient had evidence of coinfection with e. chaffeensis and a spotted fever group rick ...199910438390
antibodies to spotted fever-group rickettsiae in dogs in north carolina.a seroepidemiologic survey was conducted to determine the prevalence of antibodies reactive with 4 spotted fever-group (sfg) rickettsiae in sera of dogs from various geographic regions in north carolina. serum specimens were obtained from 600 dogs, and antibody titers were determined, using microimmunofluorescence. data analysis (setting as the criterion for a positive result, a rickettsia rickettsii titer greater than or equal to 1:64) overestimated the actual prevalence of canine exposure to t ...19873118744
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