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first isolation of rickettsia helvetica from ixodes ricinus ticks in france.two rickettsial isolates recovered from ixodes ricinus ticks in puy-de-dôme (central france) were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, western blot immunoassay, restriction fragment length polymorphism analysis, and sequencing of a portion of the citrate-synthase gene. by these methods, the isolates appeared to be identical to a member of the spotted fever group rickettsiae, rickettsia helvetica. this first isolation of rickettsia helvetica in france has ep ...19989629973
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