| [the application of pcr to epidemiological study on spotted fever group rickettsiae]. | it was the first time that a primer pairs derived from the 190kda protein antigen gene of r. rickettsii were used to amplify sfgr dna in ticks, tick ova, larva, tick faeces and rodent organs which were collected in hebei, heilongjiang, hainan and beijing. a 532bp fragment was respectively amplified from above samples. the results were partially in concordance with data obtained through rickettsiae isolation. it was suggested that pcr is a rapid, specific, sensitive and practical method for detec ... | 1995 | 7767922 |
| detection of spotted fever group rickettsiae in ticks and rodents by polymerase chain reaction technique in people's republic of china. | the polymerase chain reaction (pcr) technique for amplification of genomic fragments of spotted fever group (sfg) rickettsiae directly from field samples of ticks, tick ova, tick larvae, tick faeces and organs of wild mice was employed for the first time in p.r. of china. ticks and rodents were collected in beijing and heilongjiang, hainan and hebei provinces. the pcr primers were designed from the dna sequence encoding the 190 k protein of r. rickettsii for a 532 bp long product. seven of ten t ... | 1995 | 8722295 |
| detection of spotted fever group rickettsia in haemaphysalis longicornis in hebei province, china. | abstract dna samples from 737 ticks pools, representing 6850 haemaphysalis longicornis and 51 dermacentor nuttalli ticks collected from hebei province, china, were analyzed by pcr for the presence of spotted fever group rickettsia. there were 50 (6.9%) of 724 h. longicornis ticks pools found positive, but no positive samples found in 13 d. nuttalli. sequence analysis of the partial outer membrane protein a (ompa) genes from the ten positive samples showed 98% identity, but different from the hom ... | 2011 | 21506802 |