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failure to detect bovine viral diarrhea virus in necropsied farm-raised white-tailed deer (odocoileus virginianus) in pennsylvania.between january 1 and december 31, 2005 gross and histologic examinations were performed on carcasses of 61 farm-raised white-tailed deer originating from pennsylvania. single-tube real-time reverse transcription polymerase chain reaction (real-time rt-pcr) for the detection of bovine viral diarrhea virus type 1 (bvdv-1) and type 2 (bvdv-2) was performed on each animal. virus isolation was performed on tissue samples from 25 of 61 animals. immunohistochemical (ihc) staining of ear-notch skin to ...200717459862
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