| increased phylogenetic diversity of bovine viral diarrhoea virus type 1 isolates in england and wales since 2001. | currently, there are two recognised genotypes of bovine viral diarrhoea virus (bvdv), type 1 and type 2. these genotypes are divided into subtypes based on phylogenetic analysis, namely a-p for bvdv-1 and a-c for bvdv-2. within this study, the genetic heterogeneity of bvdv-1 in england and wales was investigated and compared to the situation in 1996/1997. viral rna was extracted from 316 blood samples collected between 2004 and 2009 that were previously identified as bvdv-1 positive. a region of ... | 2013 | 23022681 |
| genetic typing of bovine pestiviruses from england and wales. | using rna purified directly from stored clinical specimens, a collection of 62 pestiviruses were typed by rt-pcr and sequencing within the 5'-untranslated region of the genome. all the specimens had been obtained in 1966/1967 from diary cattle in england and wales. eight further pestiviruses, grown in cell culture, were characterised in the same way. seven of these viruses were representatives of a panel of british isolates, obtained from cattle ten years before. the eighth was the virus used in ... | 1999 | 10535769 |