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experimental infection of vertebrates of the pocomoke cypress swamp, maryland with keystone and jamestown canyon viruses.experimental studies were conducted to assess the susceptibility of white-tailed deer (odocoileus virginianus), gray squirrels (sciurus carolinensis), and cottontail rabbits (sylvilagus floridanus) to jamestown canyon (jc) and/or keystone (key) virus infection. viremia occurred in 5 of 6 deer inoculated with jc virus; however, all deer developed key virus neutralizing antibody. based on the observation that antibody elicited by primary infection of deer with either key or jc virus exhibited part ...1979453437
trivittatus virus infections in wild mammals and sentinel rabbits in central iowa.a serological survey was conducted in iowa to determine the prevalence rate of california group virus antibodies in sera of several vertebrate species. serum specimens were assayed for infectivity-neutralizing antibody in a microneutralization system with baby hamster kidney cell culture. of 77 sera assayed, 21 (27%) neutralized trivittatus (tvt) virus infectivity. the antibody prevalence rate was highest for eastern cottontail rabbits inasmuch as 46% (10/22) of the serum specimens form this spe ...19751200251
incrimination of aedes provocans as a vector of jamestown canyon virus in an enzootic focus of northeastern new york.a 2-year field study was conducted in southern saratoga county, new york, to determine which species of the aedes communis group mosquitoes were potential vectors of jamestown canyon (jc) virus. a total of 23,890 mosquitoes (890 pools) were processed for virus isolation in 1988-89, yielding 17 jc virus isolates from ae. provocans and one isolate each from ae. communis, ae. intrudens and ae. punctor. minimum field infection rates (mfir) and daily mfirs as high as 1:219 and 1:38, respectively, wer ...19902230781
infection of white-tailed deer (odocoileus virginianus) in michigan with jamestown canyon virus (california serogroup) and the importance of maternal antibody in viral maintenance.sera collected from a captive population of white-tailed deer (odocoileus virginianus) penned in the lower peninsula of michigan were assayed over a 29-mo period for neutralizing antibody to california serogroup viruses. in all, 130 individual white-tailed deer were bled one to 22 times between june 1983 and november 1985. of the 130 sampled after active transmission had ceased, or passage of maternal antibody in colostrum had occurred, only one (0.8%), a newborn fawn, had no serum neutralizing ...19873102763
prevalence of neutralizing antibody to jamestown canyon virus (california group) in populations of elk and moose in northern michigan and ontario, canada.blood samples were collected from free-ranging elk (cervus elaphus) harvested in michigan's northern lower peninsula, from moose (alces alces) relocated from ontario's algonquin provincial park to michigan's upper peninsula, and from moose from michigan's isle royale national park. sera were tested by serum dilution neutralization tests in vero cell culture for neutralizing antibody to california serogroup viruses, in particular jamestown canyon (jc), la crosse/snowshoe hare (lac/ssh), and trivi ...19863503129
california serogroup viruses in new york state: the role of subtypes in human infections.the roles of various subtypes of the california serogroup viruses as infectious agents and as neuropathogens were evaluated by using the plaque reduction neutralization test. sera from 394 patients with central nervous system (cns) infections during 1971-1982 and from 501 persons without cns manifestations were studied. jamestown canyon (jc) and la crosse (lac) viruses were found to have been common infectious agents in new york state for at least 16 years. jc virus was the prevalent indicated a ...19846507732
mosquito and arbovirus surveillance in connecticut, 1991-1992.a surveillance program for mosquito-borne arboviruses was conducted in connecticut following an epizootic of eastern equine encephalitis (eee) in horses and domestic birds during 1990. mosquito trapping was done weekly using co2-baited miniature light traps at 12 freshwater swamp sites that were located mostly in the southeastern portion of the state. trapping was conducted from june 27 to october 11, 1991 and from june 2 to september 30, 1992. totals of 7,435 (1991) and 13,912 (1992) adult fema ...19947707064
isolations of jamestown canyon virus (bunyaviridae: california serogroup) from mosquitoes (diptera: culicidae) in the western united states, 1990-1992.nearly 80,000 immature and adult mosquitoes in three genera were collected in high-elevation (> 1,000 m) areas of california (68,229), nevada (3,721), oregon (5,918), and washington (1,629) during 1990-1992 and tested for virus as adult males or females in 1,799 pools. collections comprised primarily alpine aedes in the aedes communis (de geer) group of the subgenus ochlerotatus. thirteen strains of jamestown canyon (jc) virus were recovered by plaque assay in vero cell culture from three member ...19937903698
ecology of jamestown canyon virus (bunyaviridae: california serogroup) in coastal california.this paper reports the first isolation of jamestown canyon (jc) virus from coastal california and the results of tests for antibody to jc virus in mammals living in coastal california. the virus isolation was made from a pool of 50 aedes dorsalis females collected as adults from morro bay, san luis obispo county, california. the virus isolate was identified by two-way plaque reduction-serum dilution neutralization tests done in vero cell cultures. sera from the mammals were tested for antibody t ...19968780458
serologic evidence of jamestown canyon virus infection in white-tailed deer populations from connecticut.we determined the prevalence and distribution of jamestown canyon (jc) virus antibody in white-tailed deer (odocoileus virginianus) populations in connecticut, usa. sera were collected from hunter-killed deer during 1993. antibody to jc virus was detected by enzyme-linked immunosorbent assay (elisa) in 92 (21%) of 446 deer sera, and was uniformly distributed among geographic sites. twenty-one elisa-positive sera were tested and confirmed positive by plaque reduction neutralization testing. this ...19979249710
vector competence of alpine, central valley, and coastal mosquitoes (diptera: culicidae) from california for jamestown canyon virus.mosquitoes collected from alpine, central valley, and coastal habitats in california were evaluated for their vector competence for four strains of jamestown canyon (jc) virus. three of the viral strains examined were isolated from alpine aedes species collected in california, and one, the prototype jc virus, was isolated from culiseta inornata (williston) collected in colorado. alpine aedes tahoensis dyar, ae. cataphylla dyar, ae. hexodontus dyar, ae. increpitus dyar, ae. clivis lanzaro and eld ...19938459417
serologic evidence of jamestown canyon and keystone virus infection in vertebrates of the delmarva peninsula.serological data accumulated during the past decade indicated that a variety of feral and domestic animals of the delaware-maryland-virginia (delmarva) peninsula were infected with jamestown canyon (jc) and/or keystone (key) viruses (bunyaviridae, california serogroup). neutralizing (n) antibody to jc virus was most prevalent in white-tailed deer, sika deer, cottontail rabbits and horses. key virus n antibody was detected most frequently in gray squirrels and domestic goats. n antibody indicativ ...19827149110
la crosse encephalitis: occurrence of disease and control in a suburban area.fifteen (6.4%) of 233 residents sampled in state road coulee (src) during 1972-73 had antibodies neutralizing la crosse (lac) virus, 9 (3.9%) trivittatus (tvt), 12 (5.2%) jamestown canyon (jc) and 2 (0.9%) bunyamwera serogroup virus. six of seven youths who had antibodies to lac virus had been ill: three were serologically confirmed as cases of lac encephalitis and three had possibly related illnesses. the other reported no illness. possibly related illnesses were reported by 1 of 10 adults with ...19836867036
oral transmission of jamestown canyon virus by aedes provocans mosquitoes from northeastern new york.aedes provocans were allowed to feed on a bloodmeal containing 5.6 log10 tcid50/ml of jamestown canyon (jc) virus. after 14 days of incubation at 21 degrees c and 80% rh, 100% (36/36) were midgut infected, 50% (18/36) developed disseminated infections and 50% (9/18) of the latter specimens transmitted virus to capillary tubes. when mosquitoes were intrathoracically inoculated with 6.1 log10 tcid50/ml of jc virus, 100% (40/40) became disseminated infected and 95% (38/40) transmitted virus after 1 ...19911675258
progressive multifocal leukoencephalopathy in persons infected with human immunodeficiency virus, san francisco, 1981-1989.progressive multifocal leukoencephalopathy (pml), a rare neurological disease, has been sporadically reported in persons infected with human immunodeficiency virus (hiv), the causative agent of acquired immune deficiency syndrome (aids). from january 1981 through february 1989, in san francisco, we identified 94 hiv-infected persons with pml, of whom 48 (51%) were pathologically confirmed (as required for aids case reporting). these 48 patients were significantly older when diagnosed with aids ( ...19911665053
natural transmission of keystone virus to sentinel rabbits on the delmarva peninsula.domestic rabbits were exposed in the pocomoke cypress swamp from early may through october 1973, to act as sentinel indicators of keystone (key) virus transmission on the delaware-maryland-virginia (delmarva) peninsula. rabbits were bled weekly and their sera were tested for the presence of neutralizing antibody to both key and james-town canyon (jc) viruses. adult mosquito activity was monitored concurrently by cdc miniature light traps with dry ice. of 100 rabbits exposed, 20 rabbits acquired ...1978717629
genetic diversity of human polyomavirus jcpyv in southern california wastewater.jc polyomavirus (jcpyv) has the potential to be used as a viral marker for human waste contamination because at least 40% of the human population excretes this virus through its urine. in addition, each of 6 known subtypes of jcpyv is associated with a specific human ethnicity group, which has allowed for tracing of human migration. this study aims to explore the possibility of linking the genetic diversity of jcpyv with source of human waste. primary sewage effluent from irvine ranch water dist ...200818475011
occurrence of the european subgroup of subtype i bk polyomavirus in japanese-americans suggests transmission outside the family.to examine the mode of transmission of bk polyomavirus (bkv), urine samples were collected from japanese-americans in los angeles and from other southern californians. subtype i was the main subtype found in samples from both groups. the subtype i subgroup ib-2, which is predominant in europe, was the primary subgroup detected in second-generation japanese-americans and in southern californians; however, the ic subgroup prevalent in native japanese was rare in these populations. since the europe ...200717855530
prevalence of antibodies against saint louis encephalitis and jamestown canyon viruses in california horses.jamestown canyon (jc) and saint louis encephalitis (sle) viruses are mosquito-transmitted viruses that have long been present in california. the objective of this study was to determine the seroprevalence of these two viruses in horses prior to the introduction of west nile (wn) virus. approximately 15% of serum samples collected in 1998 from 425 horses on 44 equine operations horses throughout california had serum antibodies to jc virus, whereas antibodies were not detected to sle virus. the re ...200415001316
asian genotypes of jc virus in japanese-americans suggest familial transmission.to examine the mode of jc virus (jcv) transmission, we collected urine samples from second- and third-generation japanese-americans in los angeles, calif., whose parents and grandparents were all japanese. from the urine samples of these japanese-americans, we mainly detected two subtypes (cy and my) of jcv that are predominantly found among native japanese. this finding provides support for the hypothesis that jcv is transmitted mainly within the family through long-term cohabitation.200212208989
jc virus type 2b is found more frequently in brain tissue of progressive multifocal leukoencephalopathy patients than in urine from controls.previous studies have shown that strains of human polyomavirus jc (jcv) of asian origin (type 2) are much more highly represented in progressive multifocal leukoencephalopathy (pml) brain than would be expected from their frequency of excretion in urine samples of a comparable control group. the present studies were designed to test whether one subtype of type 2 was preferentially elevated.199810195243
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