| identification of treponema pallidum subspecies pallidum in a 200-year-old skeletal specimen. | treponema pallidum subsp. pallidum, the causative agent of venereal syphilis, was detected in a 200-year-old skeletal specimen from easter island. an initial diagnosis of treponemal infection was confirmed by extensive purification of immunoglobulin that reacted strongly with t. pallidum antigen. extracted dna exhibited a single-base polymorphism that distinguished t.p. subsp. pallidum from 4 other human and nonhuman treponemes. extensive precautions against contamination of the subject matter w ... | 1999 | 10558971 |