| detection of spotted fever group rickettsiae in ticks and rodents by polymerase chain reaction technique in people's republic of china. | the polymerase chain reaction (pcr) technique for amplification of genomic fragments of spotted fever group (sfg) rickettsiae directly from field samples of ticks, tick ova, tick larvae, tick faeces and organs of wild mice was employed for the first time in p.r. of china. ticks and rodents were collected in beijing and heilongjiang, hainan and hebei provinces. the pcr primers were designed from the dna sequence encoding the 190 k protein of r. rickettsii for a 532 bp long product. seven of ten t ... | 1995 | 8722295 |
| genotypic identification of three new strains of spotted fever group rickettsiae isolated in china. | polymerase chain reaction (pcr) and restriction endonuclease fragment length polymorphism (rflp) analysis were used to characterize the genotypic diversity of three isolates of spotted fever group (sfg) rickettsiae isolated from ticks in china. a primer pair designed from dna sequence encoding 190 k protein antigen of r. rickettsii and genomic dnas obtained from the isolates were used in pcr. the pcr products were cleaved with restriction endonucleases psti and rsai, and the digestion patterns w ... | 1996 | 9014012 |
| [detection of north-asia tick-borne spotted fever in ticks and rodents along the heilongjiang river-side by restriction fragment length polymorphism of pcr products]. | in order to elucidate the natural foci of north-asia tick-borne spotted fever along the bank of heilongjiang river, we used pcr/rflp to detect spotted fever group rickettsiae in ticks and rodents. the results showed that the wild samples of dermacentor silvarum, haemaphysalis concinna and apodemus agrarius, microtus fortis, clethrionomys rufocanus and ondatra zibethica were all positive with amplification, but typhus rickettsiae, tsutsugamushi fever rickettsiae and q fever rickettsiae were all n ... | 1997 | 9812471 |
| natural anaplasma phagocytophilum infection of ticks and rodents from a forest area of jilin province, china. | polymerase chain reaction integrated with sequence analysis was carried out to investigate the natural anaplasma phagocytophilum infection in ticks and rodents from a forest area of jilin province, china. four (4.0%) of 100 ixodes persulcatus and 2 (0.7%) of 286 dermacentor silvarum ticks collected by flagging vegetation were positive. nine (8.8%) of 102 rodents were infected, as well as 2 (2.8%) of 71 i. persulcatus parasitizing on 25 rodents. the nucleotide sequences of 1442-bp a. phagocytophi ... | 2006 | 17038691 |
| [investigation on the vectors of borrella burgdorferi and on the identification of the isolates along china-russia border in eastern heilongjiang province, china]. | to explore the fact that the east border of heilongjiang had been a lyme disease natural focus,we investigated the species and distribution of ticks and isolated bacteria from ticks and identified genomic species of borrelia burdorferi sensu lato. this study provided evidence for prevention and control of lyme disease. | 2007 | 17575937 |
| detection of francisella tularensis in ticks and identification of their genotypes using multiple-locus variable-number tandem repeat analysis. | tularemia was reported in china over 50 years ago, however, many epidemical characteristics remain unclear. in the present study, the prevalence of francisella tularensis in ticks was investigated during an epidemiological surveillance in china and then we measured their genetic diversity by conducting multiple-locus variable- number tandem repeat analysis (mlva). | 2008 | 18798995 |