| detection of spotted fever group rickettsiae in ticks and rodents by polymerase chain reaction technique in people's republic of china. | the polymerase chain reaction (pcr) technique for amplification of genomic fragments of spotted fever group (sfg) rickettsiae directly from field samples of ticks, tick ova, tick larvae, tick faeces and organs of wild mice was employed for the first time in p.r. of china. ticks and rodents were collected in beijing and heilongjiang, hainan and hebei provinces. the pcr primers were designed from the dna sequence encoding the 190 k protein of r. rickettsii for a 532 bp long product. seven of ten t ... | 1995 | 8722295 |
| detection of borrelia burgdorferi from ticks (acari) in hebei province, china. | from may 1992 to august 1993, 1,196 ticks were collected by flagging vegetation and from domestic animals in chengde, zhangjiakou, shijiazhuang, and handan districts of hebei province. of those ticks collected, 954 were haemaphysalis japonicum warburton and h. longicornis neumann, 205 were ixodes persulcatus schulze, and 37 were dermacentor silvarum olenev. four strains of borrelia burgdorferi were isolated from 135 i. persulcatus collected in weichang and zhuolu counties. three of 574 haemaphys ... | 1998 | 9538567 |
| detection of spotted fever group rickettsia in haemaphysalis longicornis in hebei province, china. | abstract dna samples from 737 ticks pools, representing 6850 haemaphysalis longicornis and 51 dermacentor nuttalli ticks collected from hebei province, china, were analyzed by pcr for the presence of spotted fever group rickettsia. there were 50 (6.9%) of 724 h. longicornis ticks pools found positive, but no positive samples found in 13 d. nuttalli. sequence analysis of the partial outer membrane protein a (ompa) genes from the ten positive samples showed 98% identity, but different from the hom ... | 2011 | 21506802 |
| molecular detection of anaplasma phagocytophilum in ixodid ticks in hebei province, china. | a total of 3696 ixodid ticks, collected from hebei province, china, were examined by a nested polymerase chain reaction for the presence of anaplasma phagocytophilum. forty-three (15.4%) of 280 pools tested, including 39 (14.6%) of 267 haemaphysalis longicornis and four (30.8%) of 13 dermacentor nuttalli, were positive, but no significant difference was found between d. nuttalli and h. longicornis (p>0.05). sequence and phylogenetic analyses of 16s rrna gene indicated that a. phagocytophilum in ... | 2011 | 21923254 |