pcr amplification from fixed tissue indicates frequent involvement of brachyspira aalborgi in human intestinal spirochetosis. | pcr procedures amplifying portions of the 16s rrna and nadh oxidase genes of brachyspira aalborgi and serpulina pilosicoli were applied to dna extracted from paraffin-embedded human colonic or rectal tissues from 30 norwegian, australian, and u.s. patients, 16 of whom had histologic evidence of intestinal spirochetosis (is). b. aalborgi-specific sequences were identified by pcr in 10 of the is patients (62.5%) but none of the others, while s. pilosicoli sequences were not detected in tissues fro ... | 1999 | 10325391 |