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pcr amplification from fixed tissue indicates frequent involvement of brachyspira aalborgi in human intestinal spirochetosis.pcr procedures amplifying portions of the 16s rrna and nadh oxidase genes of brachyspira aalborgi and serpulina pilosicoli were applied to dna extracted from paraffin-embedded human colonic or rectal tissues from 30 norwegian, australian, and u.s. patients, 16 of whom had histologic evidence of intestinal spirochetosis (is). b. aalborgi-specific sequences were identified by pcr in 10 of the is patients (62.5%) but none of the others, while s. pilosicoli sequences were not detected in tissues fro ...199910325391
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