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phylogenetic diversity of nitrogen-fixing bacteria in mangrove sediments assessed by pcr-denaturing gradient gel electrophoresis.culture-independent pcr-denaturing gradient gel electrophoresis (dgge) was employed to assess the composition of diazotroph species from the sediments of three mangrove ecosystem sites in sanya, hainan island, china. a strategy of removing humic acids prior to dna extraction was conducted, then total community dna was extracted using the soil dna kit successfully for nifh pcr amplification, which simplified the current procedure and resulted in good dgge profiles. the results revealed a novel ni ...200818347779
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