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pcr-based study of conserved and variable dna sequences of tritrichomonas foetus isolates from saskatchewan, canada.the protozoan parasite tritrichomonas foetus causes infertility and spontaneous abortion in cattle. in saskatchewan, canada, the culture prevalence of trichomonads was 65 of 1,048 (6%) among 1,048 bulls tested within a 1-year period ending in april 1994. saskatchewan was previously thought to be free of the parasite. to confirm the culture results, possible t. foetus dna presence was determined by the pcr. all of the 16 culture-positive isolates tested were pcr positive by a single-band test, bu ...19957615746
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