| isolation and identification of legionella and their host amoebae from weak alkaline carbonate spring water using a culture method combined with pcr. | legionella were detected with the direct dna extraction method, legionella culture method, and free-living amoebae (fla) culture method from weak alkaline carbonate spring water in taiwan. moreover, we also investigated the existence of acanthamoeba, hartmannella, and naegleria, ubiquitous fla in aquatic environments, to identify the correlations between existing legionella. this study reports detecting legionella in 15 of the 51 weak alkaline carbonate spring water samples (29.4%). this work al ... | 2011 | 21537990 |
| identification and quantification of the acanthamoeba species and genotypes from reservoirs in taiwan by molecular techniques. | the occurrence of acanthamoeba was investigated from 21 main reservoirs of taiwan with 12 (57.1%) testing positive. analysis of the 18s rrna gene pcr product was performed in order to identify the acanthamoeba isolates. acanthamoeba spp. concentrations were determined according to taqman real-time qpcr. acanthamoeba genotypes of all isolates were identified t4. the species were categorized to acanthamoeba culbertsoni, acanthamoeba polyphaga, acanthamoeba castellanii and acanthamoeba hatchetti. t ... | 2014 | 24388954 |