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molecular characterization of a novel recombinant strain of human astrovirus associated with gastroenteritis in children.we report a naturally occurring human astrovirus (hastv) strain detected in two different geographic locations. we identified two isolates of this strain in a diarrhea outbreak at a child care center in houston, texas; and two isolates in diarrhea stool samples from two children in mexico city. all four isolates were detected in stool samples by enzyme immunoassay (eia). one of the mexican isolates was typed by eia and all four isolates were hastv-5 by typing rt-pcr. the four isolates were >97% ...200111811685
molecular epidemiology of human astrovirus diarrhea among children from a periurban community of mexico city.human astroviruses (hastvs) were detected in 23 stool samples from 365 diarrhea episodes among 214 children (<18 months old) prospectively monitored for diarrhea in mexico city. stool samples were tested by eia and reverse transcriptase-polymerase chain reaction (rt-pcr) analysis. eia was less sensitive (74%) and equally specific, compared with rt-pcr analysis using type-common primers for hastv detection. of 31 hastv isolates, eia typed 18 (69%) of 26 eia-positive samples, and rt-pcr analysis t ...200111181143
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