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comparison of cultural and analytical methods for determination of aflatoxin production by mississippi delta aspergillus isolates.this study compared cultural and analytical methods for detecting aflatoxin production by aspergillus species. aspergillus isolates were obtained from various mississippi delta crops (corn, peanut, rice, cotton) and soils. most of the isolates (99%) were a. flavus and the remainder comprised a. parasiticus and a. nomius. the following three cultural methods were evaluated on potato dextrose agar: fluorescence (fl) on beta-cyclodextrin-containing media (cd), yellow pigment (yp) formation in mycel ...200415105886
population ecology of aspergillus flavus associated with mississippi delta soils.understanding the source of aspergillus flavus is required to manage aflatoxin contamination of maize (zea mays l.). studies assessed a. flavus propagules, fusarium spp., and total fungi associated with mississippi delta soils. soils from 12 and 15 sites were collected in 2000 and 2001, respectively. the propagule density of a. flavus ranged from log(10) 2.0 to 4.3 colony-forming units (cfu) g(-1) soil, while total fusaria ranged from log(10) 3.0 to 5.4 cfu g(-1) soil. the highest populations of ...200717886181
mycotoxin occurrence and aspergillus flavus soil propagules in a corn and cotton glyphosate-resistant cropping systems.the effects of cotton-corn rotation and glyphosate use on levels of soil-borne aspergillus flavus, aflatoxin and fumonisin contamination in corn and cotton seed were determined during 2002-2005 in stoneville, mississippi (usa). there were four rotation systems (continuous cotton, continuous corn, cotton-corn and corn-cotton) for both glyphosate-resistant (gr) and non-gr cultivars-herbicide system arranged in a randomized complete block design with four replications. aspergillus flavus population ...200717917911
occurrence of aflatoxin in three maize (zea mays l.) hybrids over 5 years in northern mississippi.aflatoxins are produced as secondary metabolites under conducive climatic conditions by aspergillus flavus. the incidence of aflatoxin varies with environmental conditions, genotype, and location. an expanded understanding of the interaction of the plant, fungus, and weather conditions is needed to further elucidate the field infection process of maize by a. flavus and subsequent aflatoxin contamination. one of the problems in evaluating maize hybrids for resistance to kernel infection and aflat ...200817922303
dynamics of mycotoxin and aspergillus flavus levels in aging bt and non-bt corn residues under mississippi no-till conditions.mycotoxin and aspergillus flavus levels in soil-surface corn debris left by no-till agriculture methods (stover, cobs, and cobs with grain) were determined during the december-march fallow period for near-isogenic bt and non-bt hybrid corn. by december, average mycotoxin levels in non-bt corn were many times higher in cobs with grain than in grain harvested in september (total aflatoxins, 774 vs 211 ng/g; total fumonisins, 216 vs 3.5 microg/g; cyclopiazonic acid, 4102 vs 72.2 microg/g; zearaleno ...200818642924
comparison of major biocontrol strains of non-aflatoxigenic aspergillus flavus for the reduction of aflatoxins and cyclopiazonic acid in maize.biological control of toxigenic aspergillus flavus in maize through competitive displacement by non-aflatoxigenic strains was evaluated in a series of field studies. four sets of experiments were conducted between 2007 and 2009 to assess the competitiveness of non-aflatoxigenic strains when challenged against toxigenic strains using a pin-bar inoculation technique. in three sets of experiments the non-aflatoxigenic strain k49 effectively displaced toxigenic strains at various concentrations or c ...201121259141
genetic variability of aspergillus flavus isolates from a mississippi corn field.a nontoxigenic aspergillus flavus strain, k49, is currently being tested as a biological control agent in corn fields in the mississippi delta. however, little is known about the overall genetic diversity of a. flavus from year to year in corn fields and specifically in mississippi. our objective was to assess the genetic variability of a. flavus isolates from different seasons, inoculum sources, and years, from a no-till corn field. of the 175 a. flavus isolates examined, 74 and 97 had the typi ...201425478591
characterization of expressed sequence tag-derived simple sequence repeat markers for aspergillus flavus: emphasis on variability of isolates from the southern united states.simple sequence repeat (ssr) markers were developed from aspergillus flavus expressed sequence tag (est) database to conduct an analysis of genetic relationships of aspergillus isolates from numerous host species and geographical regions, but primarily from the united states. twenty-nine primers were designed from 362 tri-nucleotide est-ssr sequences. eighteen polymorphic loci were used to genotype 96 aspergillus species isolates. the number of alleles detected per locus ranged from 2 to 24 with ...201222911544
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