| the use of nemabiome metabarcoding to explore gastro-intestinal nematode species diversity and anthelmintic treatment effectiveness in beef calves. | next-generation deep amplicon sequencing, or metabarcoding, has revolutionized the study of microbial communities in humans, animals and the environment. however, such approaches have yet to be applied to parasitic helminth communities. we recently described the first example of such a method - nemabiome sequencing - based on deep-amplicon sequencing of internal transcribed spacer 2 (its-2) rdna, and validated its ability to quantitatively assess the species composition of cattle gastro-intestin ... | 2017 | 28797791 |
| host specificity of sheep and cattle nematodes in são paulo state, brazil. | the trial was carried out to investigate parasite host specificity and to analyse the dynamics of infection with nematodes parasitizing sheep and cattle raised together or separately in são paulo state, brazil, and, also to clarify doubts about the systematics of species of the genus haemonchus on the basis of cytological and morphological studies. ten steers and 32 ewes were randomly assigned to three paddocks (p), as follows: p1, 5 steers; p2, 5 steers and 16 ewes; and p3, 16 ewes. the animals ... | 1997 | 9477496 |