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prevalence and risk factor analysis of bovine hemoplasma infection by direct pcr in eastern hokkaido, japan.the prevalence and risk factors of 2 bovine hemoplasma species-mycoplasma wenyonii (mw) and "candidatus mycoplasma haemobos" (cmh)-were examined by direct pcr using whole blood samples collected from 343 cattle in eastern hokkaido, japan. the sensitivity of the direct pcr assay was 10-fold higher than the standard pcr assay, detecting 5 copies of bovine hemoplasma dna per reaction. an epidemiological survey of sampling location, living conditions, age and bovine leukemia virus antibody was perfo ...201222673725
molecular detection of mycoplasma wenyonii and 'candidatus mycoplasma haemobos' in cattle in hokkaido, japan.blood samples from 78 cattle were tested for hemoplasma infection using molecular methods. pcr and sequence analysis revealed that 17 cattle were infected with mycoplasma wenyonii, while 13 were infected with 'candidatus mycoplasma haemobos'. four animals were infected with both species. this is the first study to report hemoplasma species infection among cattle in japan.200818571343
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