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assessment of polymicrobial infections in ticks in new york state.ixodes scapularis ticks are clinically important hematophagous vectors. a single tick bite can lead to a polymicrobial infection. we determined the prevalence of polymicrobial infection with borrelia burgdorferi, anaplasma phagocytophilum, babesia microti, borrelia miyamotoi, and powassan virus in 286 adult ticks from the two counties in new york state where lyme disease is endemic, utilizing a masstag multiplex polymerase chain reaction assay. seventy-one percent of the ticks harbored at least ...201019725770
prevalence of borrelia miyamotoi in ixodes ticks in europe and the united states.borrelia miyamotoi, a relapsing fever-related spirochete transmitted by ixodes ticks, has been recently shown to be a human pathogen. to characterize the prevalence of this organism in questing ixodes ticks, we tested 2,754 ticks for a variety of tickborne pathogens by pcr and electrospray-ionization mass spectrometry. ticks were collected from california, new york, connecticut, pennsylvania, and indiana in the united states and from germany and the czech republic in europe from 2008 through 201 ...201425280366
detection of borrelia miyamotoi and other tick-borne pathogens in human clinical specimens and ixodes scapularis ticks in new york state, 2012-2015.borrelia miyamotoi (bm) is a recently emerging bacterial agent transmitted by several species of ixodid ticks. diagnosis of bm infection can be challenging, as the organism is not easily cultivable. we have developed and validated a multiplex real-time pcr to simultaneously identify bm infection and the agents causing human granulocytic anaplasmosis and human monocytic ehrlichiosis, anaplasma phagocytophilum and ehrlichia chaffeensis, respectively. the assay is 100% specific; highly sensitive, d ...201728131594
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