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isolation in cell cultures and initial characterisation of two novel bocavirus species from swine in northern ireland.we report the isolation in cell cultures of two novel bocavirus species in pigs from farms in northern ireland with clinical postweaning multisystemic wasting syndrome (pmws). we have designated the isolates as porcine bocavirus-3 (pbov3) and porcine bocavirus-4 (pbov4). to date 5082 and 4125bps of pbov3 and pbov4 have been sequenced, respectively. pbov3 and pbov4 show nucleotide homology to other known bocaviruses in swine and other organisms. open reading frame (orf) analysis has shown that th ...201121605951
production, characterisation and applications of monoclonal antibodies to two novel porcine bocaviruses from swine in northern ireland.the production, preliminary characterisation and applications of monoclonal antibodies (mabs) against two novel swine bocaviruses isolated in cell culture from swine in northern ireland are described. of the 17 stable final clones produced, four were characterised. all were of the igg2a isotype and showed no cross-reactivity with either bocavirus strain. partial neutralisation was observed with pbov4 mabs and homologous virus. the two mabs selected for use in antigen-detecting elisas were succes ...201121947503
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