| identification of unique dna sequences present in highly virulent 2009 alabama isolates of aeromonas hydrophila. | in 2009, a disease outbreak caused by aeromonas hydrophila occurred in 48 catfish farms in west alabama, causing an estimated loss of more than 3 million pounds of food size channel catfish. virulence studies have revealed that the 2009 isolates of a. hydrophila are at least 200-fold more virulent than a 1998 alabama isolate al98-c1b. however, up to now, no molecular markers have been identified to differentiate the highly virulent 2009 isolates from other isolates of a. hydrophila. to understan ... | 2011 | 21561726 |
| molecular identification and virulence of three aeromonas hydrophila isolates cultured from infected channel catfish during a disease outbreak in west alabama (usa) in 2009. | three isolates (al09-71, al09-72, and al09-73) of aeromonas hydrophila were cultured from infected channel catfish ictalurus punctatus during a disease outbreak in west alabama, usa, in august 2009. sequence analysis of the 16s-23s rdna intergenic spacer region (isr), cpn60, gyrb, and rpod genes of the 3 strains revealed that the 3 strains were closely related to each other, sharing 97 to 99% nucleotide sequence similarities. however, isr sequences of the 3 isolates from 2009 shared only 64% nuc ... | 2011 | 21790073 |
| development and efficacy of novobiocin and rifampicin-resistant aeromonas hydrophila as novel vaccines in channel catfish and nile tilapia. | three attenuated aeromonas hydrophila vaccines were developed from the virulent 2009 west alabama isolates through selection for resistance to both novobiocin and rifampicin. when channel catfish (ictalurus punctatus) were ip injected with 4×105 colony-forming unit (cfu) of the mutants, no fish died. however, when the same age and size matched channel catfish were ip injected with similar amount of their virulent parents, 80-100% fish died. similarly, when nile tilapia (oreochromis niloticus) we ... | 2011 | 21872628 |
| farm size, seining practices, and salt use: risk factors for aeromonas hydrophila outbreaks in farm-raised catfish, alabama, usa. | in freshwater aquaculture systems, aeromonas hydrophila is usually considered to be an opportunistic pathogen most often associated with secondary bacterial infections. since 2009, the u.s. catfish industry, especially in west alabama, has been affected by mortality from a strain of a. hydrophila that has been acting as a primary pathogen. tens of millions of pounds of catfish production have been lost as a consequence of this disease. this study used data from two whole-population farmer survey ... | 2015 | 25466218 |
| an asian origin of virulent aeromonas hydrophila responsible for disease epidemics in united states-farmed catfish. | since 2009, catfish farming in the southeastern united states has been severely impacted by a highly virulent and clonal population of aeromonas hydrophila causing motile aeromonas septicemia (mas) in catfish. the possible origin of this newly emerged highly virulent a. hydrophila strain is unknown. in this study, we show using whole-genome sequencing and comparative genomics that a. hydrophila isolates from diseased grass carp in china and catfish in the united states have highly similar genome ... | 2014 | 24895303 |
| rapid quantitative detection of aeromonas hydrophila strains associated with disease outbreaks in catfish aquaculture. | a new strain of aeromonas hydrophila has been implicated in significant losses in farm-raised catfish. outbreaks attributable to this new strain began in alabama in the summer of 2009 and have spread to arkansas and mississippi in subsequent years. these outbreaks mostly afflicted market-sized fish and resulted in considerable losses in short periods of time. the present research was designed to develop an expeditious diagnostic procedure to detect the new strains of a. hydrophila due to the rap ... | 2013 | 23847227 |
| identification, virulence, and mass spectrometry of toxic ecp fractions of west alabama isolates of aeromonas hydrophila obtained from a 2010 disease outbreak. | in west alabama, disease outbreaks in 2009 caused by aeromonas hydrophila have led to an estimated loss of more than $3 million. in 2010, disease outbreak occurred again in west alabama, causing losses of hundreds of thousands of pounds of market size channel catfish. during the 2010 disease outbreak in west alabama, four isolates of a. hydrophila were cultured from the kidney tissues of diseased channel catfish. both analytical profile index (api) 20 e biochemical tests and 16s-23s rrna sequenc ... | 2013 | 23523171 |
| lesions caused by virulent aeromonas hydrophila in farmed catfish ( ictalurus punctatus and i. punctatus × i. furcatus) in mississippi. | in 2009, a virulent strain of aeromonas hydrophila caused severe disease and high mortalities (motile aeromonad septicemia variant [masv]) in farmed channel catfish ( ictalurus punctatus) and hybrid catfish ( i. punctatus × i. furcatus) in eastern mississippi and alabama. as is common in mas, there is severe hemorrhagic dermatitis with ulceration, as well as abdominal hyperemia, petechiation, and mild ascites. additional findings in masv cases include panophthalmitis and orbital cellulitis, lead ... | 2017 | 28482758 |