| detection of infectious bursal disease viruses in commercially reared chickens using the reverse transcriptase/polymerase chain reaction-restriction endonuclease assay. | bursa samples from the united states, mexico, and puerto rico were tested for the presence of infectious bursal disease virus (ibdv) using the reverse transcriptase/polymerase chain reaction-restriction endonuclease (rt/pcr-re) assay. this assay amplifies a 394-bp fragment of the ibdv vp2 gene. a total of 151 samples were tested. each was from a different physical location or farm. forty-eight of the samples were determined to contain ibdv using rt/pcr. the re profiles on 44 of these positive sa ... | 1997 | 9087330 |