| prevalence of lyme borreliosis spirochetes in ixodid ticks of japan, with special reference to a new potential vector, ixodes ovatus (acari: ixodidae). | to determine the vectors of lyme borreliosis in japan, the ixodid ticks taken in hokkaido, fukushima, nagano, ehime, and kochi prefectures were examined individually for spirochetal infections by culturing their midgut tissues in bsk medium. the cultivable spirochetes identified as borrelia burgdorferi were detected in ixodes persulcatus schulze adults (15.5% of 634), nymph (6.7% of 15) and ixodes ovatus neumann adult (9.6% of 188) from hokkaido, i. ovatus adults (25.5% of 47) from fukushima, an ... | 1992 | 1495032 |
| detection of rickettsial dna in ixodid ticks recovered from dogs and cats in japan. | dna from ticks recovered from 1137 dogs and 133 cats from all over japan were examined for rickettsia infection by citrate synthase gene (glta)-based pcr and partial nucleotide sequencing. a total of 91 dog tick samples and 18 cat tick samples showed a single band of the appropriate size in the nested pcr. sequence analysis was successfully performed on 102 samples. dna of rickettsia japonica or closely related rickettsia spp. strains were detected from 38 ticks in 16 prefectures mainly in weste ... | 2005 | 16397379 |
| detection of dna of 'candidatus mycoplasma haemominutum' and spiroplasma sp. in unfed ticks collected from vegetation in japan. | dna fragments of 'candidatus mycoplasma haemominutum', a feline heamobartonella pathogen, were detected from unfed ixodes ovatus collected from vegetation in hokkaido, fukushima and yamaguchi prefectures, and unfed haemaphysalis flava in yamaguchi prefecture. this finding suggests that ixodid tick is a possible vector of 'c. mycoplasma haemominutum'. spiroplasma dna was also detected from unfed i. ovatus in hokkaido, fukushima and yamaguchi prefectures. the analysis of nucleotides sequence sugge ... | 2005 | 16397391 |
| molecular survey of anaplasma and ehrlichia infections of feral raccoons (procyon lotor) in hokkaido, japan. | infection by anaplasma and ehrlichia in feral raccoons (procyon lotor) in hokkaido, japan, was examined by molecular methods. a polymerase chain reaction (pcr) screen for anaplasmataceae, based on 16s rrna, showed that 38 (5.4%) of 699 raccoons examined were positive. these 38 positive samples were examined for anaplasma phagocytophilum, anaplasma bovis, ehrlichia chaffeensis, and ehrlichia canis infection by species-specific nested pcr. nested pcr results indicated that 36 of the 38 samples wer ... | 2011 | 21395415 |
| detection of anaplasma bovis and anaplasma phagocytophilum dna from haemaphysalis megaspinosa in hokkaido, japan. | dna from 101 ticks, including haemaphysalis megaspinosa, haemaphysalis douglasi, haemaphysalis flava, ixodes ovatus and ixodes persulcatus, collected by flagging in a pasture in hidaka district (hokkaido, japan) was examined for infection with anaplasma bovis and a. phagocytophilum by species-specific nested pcr. of the 48 h. megaspinosa nymphs, examined, 1 (2.1%) and 6 (12.5%) were positive for a. bovis and a. phagocytophilum, respectively. one of the 6 positive nymphs was dual positive for bot ... | 2010 | 19897306 |