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detection of emerging vaccine-related polioviruses by deep sequencing.oral poliovirus vaccine can mutate to regain neurovirulence. to date, evaluation of these mutations has been performed primarily on culture-enriched isolates by using conventional sanger sequencing. we therefore developed a culture-independent, deep-sequencing method targeting the 5' untranslated region (utr) and p1 genomic region to characterize vaccine-related poliovirus variants. error analysis of the deep-sequencing method demonstrated reliable detection of poliovirus mutations at levels of ...201728468861
use of a novel real-time pcr assay to detect oral polio vaccine shedding and reversion in stool and sewage samples after a mexican national immunization day.during replication, oral polio vaccine (opv) can revert to neurovirulence and cause paralytic poliomyelitis. in individual vaccinees, it can acquire specific revertant point mutations, leading to vaccine-associated paralytic poliomyelitis (vapp). with longer replication, opv can mutate into vaccine-derived poliovirus (vdpv), which causes poliomyelitis outbreaks similar to those caused by wild poliovirus. after wild poliovirus eradication, safely phasing out vaccination will likely require global ...201121411577
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