Publications

detection of emerging vaccine-related polioviruses by deep sequencing.	oral poliovirus vaccine can mutate to regain neurovirulence. to date, evaluation of these mutations has been performed primarily on culture-enriched isolates by using conventional sanger sequencing. we therefore developed a culture-independent, deep-sequencing method targeting the 5' untranslated region (utr) and p1 genomic region to characterize vaccine-related poliovirus variants. error analysis of the deep-sequencing method demonstrated reliable detection of poliovirus mutations at levels of ...	2017	28468861
use of a novel real-time pcr assay to detect oral polio vaccine shedding and reversion in stool and sewage samples after a mexican national immunization day.	during replication, oral polio vaccine (opv) can revert to neurovirulence and cause paralytic poliomyelitis. in individual vaccinees, it can acquire specific revertant point mutations, leading to vaccine-associated paralytic poliomyelitis (vapp). with longer replication, opv can mutate into vaccine-derived poliovirus (vdpv), which causes poliomyelitis outbreaks similar to those caused by wild poliovirus. after wild poliovirus eradication, safely phasing out vaccination will likely require global ...	2011	21411577