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rapid detection of polioviruses in environmental water samples by one-step duplex rt-pcr.this study describes the rapid detection of polioviruses in environmental waters by a simple reverse transcriptase-polymerase chain reaction (rt-pcr) using two primer pairs for differentiation of poliovirus from non-polio enteroviruses in a single reaction by a one-step method, combining rt and pcr in a single tube. the detection by agarose gel electrophoresis yielded 2 bands of 153-bp and 293-bp for poliovirus tested without the need for further hybridization. the detection sensitivity of this ...200011023064
surveillance of enterovirus infections in bangkok. i. isolation of enterovirus from faecal specimens of healthy children.during the period august 1970 through july 1973, about 50 faecal specimens collected each month from healthy pre-school children of lower economic status in bangkok were studied for virus isolation using primary monkey kidney cells and new born mice. from a total of 1,823 samples, 557 (30.5%) were positive: 86 (4.8%) polioviruses, 77 (4.2%) coxsackieviruses b, 223 (12.2%) echoviruses, and 127 strains (7.0%) of unidentified viruses. altogether, 45 serotypes of enteric viruses were identified. mor ...1975167453
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