detection of viral pathogens by reverse transcriptase pcr and of microbial indicators by standard methods in the canals of the florida keys. | in order to assess the microbial water quality in canal waters throughout the florida keys, a survey was conducted to determine the concentration of microbial fecal indicators and the presence of human pathogenic microorganisms. a total of 19 sites, including 17 canal sites and 2 nearshore water sites, were assayed for total coliforms, fecal coliforms, escherichia coli, clostridium perfringens, enterococci, coliphages, f-specific (f(+)) rna coliphages, giardia lamblia, cryptosporidium parvum, an ... | 1999 | 10473424 |
prevalence of giardia duodenalis genotypes in pre-weaned dairy calves. | to determine the prevalence of giardia genotypes in pre-weaned dairy calves, fecal samples were collected from a minimum of 18, 1-7-week-old dairy calves per farm on two farms each in the states of vermont, new york, pennsylvania, maryland, virginia, north carolina, and florida. samples cleaned of fecal debris and concentrated using cscl density gradient centrifugation were stained and examined by immunofluorescence microscopy and also subjected to pcr and gene sequence analysis. prevalence by p ... | 2004 | 15381298 |
prevalence and genotypes of giardia duodenalis in post-weaned dairy calves. | to determine the prevalence of giardia genotypes in post-weaned dairy calves, fecal specimens were collected from 3 to 11-month-old dairy calves per farm on two farms in vermont, new york, pennsylvania, maryland, virginia, north carolina, and florida. specimens cleaned of fecal debris and concentrated using cscl density gradient centrifugation were stained and examined by immunofluorescence microscopy and also subjected to pcr and dna sequence analysis. overall, pcr provided more sensitive detec ... | 2005 | 15925721 |
prevalence and genotypes of giardia duodenalis in 1-2 year old dairy cattle. | to determine the prevalence of giardia genotypes in 12-24 month old dairy heifers, fecal specimens were collected from two farms each in vermont, new york, pennsylvania, maryland, virginia, north carolina, and florida. specimens, cleaned of fecal debris and concentrated using cscl density gradient centrifugation, were subjected to pcr and dna sequence analysis. prevalence of giardia infection, ranged from 11% to 75% on 14 farms with an average prevalence of 36% (204 positive cattle out of 571 ex ... | 2006 | 16647818 |