| prevalence and molecular characterization of giardia duodenalis and cryptosporidium spp. in dairy cattle in ontario, canada. | giardia duodenalis and cryptosporidium spp. are intestinal protozoan parasites that infect a wide range of host species, including humans. molecular characterization of these parasites has demonstrated that a number of genotypes and species are common to both humans and animals, and that zoonotic transmission may occur. numerous studies have reported a high prevalence of g. duodenalis and cryptosporidium spp. in cattle, particularly calves, and these animals are frequently associated with zoonot ... | 2007 | 17964724 |
| detection of cyclospora, cryptosporidium, and giardia in ready-to-eat packaged leafy greens in ontario, canada. | numerous foodborne outbreaks of diarrheal illness associated with the consumption of produce contaminated with protozoan parasites have been reported in north america in recent years. the present study reports on the presence of cyclospora, cryptosporidium, and giardia in precut salads and leafy greens purchased at retail in ontario, canada. a total of 544 retail samples were collected between april 2009 and march 2010 and included a variety of salad blends and individual leafy greens. most of t ... | 2013 | 23433379 |
| evaluation of the zoonotic potential of giardia duodenalis in fecal samples from dogs and cats in ontario. | this study determined the distribution and zoonotic potential of giardia duodenalis assemblage types among canine and feline fecal samples from ontario. the effectiveness of giardia assemblage typing methods by sequencing the genes of small subunit ribosomal rna (ssu-rrna), β-giardin (bg), glutamate dehydrogenase (gdh), and triose phosphate isomerase (tpi) was evaluated simultaneously. from 2008 to 2010, 118 canine and 15 feline giardia positive fecal samples were tested. the ssu-rrna sequencing ... | 2011 | 22654138 |
| detection and characterization of giardia duodenalis and cryptosporidium spp. on swine farms in ontario, canada. | abstract as part of the c-enternet surveillance program of the public health agency of canada, 122 pooled swine manure samples from 10 farms in ontario, canada were collected and tested for giardia and cryptosporidium. giardia duodenalis cysts and cryptosporidium spp. oocysts were detected using immunofluorescence microscopy. nested-polymerase chain reaction protocols were performed to amplify the small subunit rrna gene and the +¦-giardin gene for g. duodenalis, and the small subunit rrna gene ... | 2011 | 21675863 |
| temporal changes in the prevalence and shedding patterns of giardia duodenalis cysts and cryptosporidium spp. oocysts in a herd of dairy calves in ontario. | giardia duodenalis and cryptosporidium spp. infections, and the patterns of cyst and oocyst shedding, were observed in a herd of dairy calves in ontario over a period of 3 mo. cysts and oocysts were detected and enumerated in fecal samples using immunofluorescence microscopy; giardia and cryptosporidium dna was detected using the polymerase chain reaction. the prevalence of g. duodenalis increased during the course of the study, reaching a peak of 93.1% when calves were 43 to 54 d old, and then ... | 2010 | 21037883 |
| the potential for zoonotic transmission of giardia duodenalis and cryptosporidium spp. from beef and dairy cattle in ontario, canada. | the objective of this study was to compare the occurrence and the genotypes and species of giardia duodenalis and cryptosporidium spp. in beef and dairy cattle from farms in the regional municipality of waterloo, ontario, in an effort to determine the potential for zoonotic transmission from these animals. pooled manure samples were collected from 45 dairy cattle farms and 30 beef cattle farms. the presence of giardia cysts and cryptosporidium oocysts was determined by immunofluorescence microsc ... | 2010 | 20971563 |
| disinfection of bacillus subtilis spores with chlorine dioxide: a bench-scale and pilot-scale study. | chlorine dioxide (clo2) inactivation of bacillus subtilis atcc 19659 spores was examined at pilot-scale during periods representative of winter and summer temperature extremes at the britannia water treatment plant in ottawa, canada. in addition, bench-scale experiments using the same source water (ottawa river, ontario, canada), as well as buffered and unbuffered laboratory waters were conducted using b. subtilis spores. bench-scale inactivation of b. subtilis spores by clo2 was similar to repo ... | 2002 | 11996351 |
| giardia lamblia in children in day-care centres in southern ontario, canada, and susceptibility of animals to g. lamblia. | in 1982, giardia lamblia cysts were found in the faeces of eight of 97 children (two to five years old) in day-care centres in guelph, ontario, canada. none of the infected children showed any of the classical signs of giardiasis. the survey was repeated in 1983 in the kitchner-waterloo area, ontario. in this survey nine of 147 children were infected with g. lamblia. experimentally established giardia-free colonies of mice, hamsters, rats, cats (adults and kittens) and dogs (adults and puppies) ... | 1986 | 3726998 |