| seasonal and biogeographical patterns of gastrointestinal parasites in large carnivores: wolves in a coastal archipelago. | parasites are increasingly recognized for their profound influences on individual, population and ecosystem health. we provide the first report of gastrointestinal parasites in gray wolves from the central and north coasts of british columbia, canada. across 60 000 km(2), wolf feces were collected from 34 packs in 2005-2008. at a smaller spatial scale (3300 km(2)), 8 packs were sampled in spring and autumn. parasite eggs, larvae, and cysts were identified using standard flotation techniques and ... | 2012 | 22309976 |
| giardia spp. are commonly found in mixed assemblages in surface water, as revealed by molecular and whole-genome characterization. | giardia is the most common parasitic cause of gastrointestinal infections worldwide, with transmission through surface water playing an important role in various parts of the world. giardia duodenalis (synonyms: g. intestinalis and g. lamblia), a multispecies complex, has two zoonotic subtypes, assemblages a and b. when british columbia (bc), a western canadian province, experienced several waterborne giardiasis outbreaks due to unfiltered surface drinking water in the late 1980s, collection of ... | 2015 | 25956776 |
| multiplex assay detection of immunoglobulin g antibodies that recognize giardia intestinalis and cryptosporidium parvum antigens. | giardiasis and cryptosporidiosis are common enteric parasitic diseases that have similar routes of transmission. in this work, we have identified epitopes within the giardia variant-specific surface protein (vsp) sequences that are recognized by igg antibodies from 13 of 14 (93%) sera from patients with stool-confirmed giardiasis. the conserved epitopes are shared among vsps from both of the assemblages that commonly infect humans, and they are likely to be structural, as both sodium dodecyl sul ... | 2010 | 20876825 |
| evaluation of molecular techniques to biotype giardia duodenalis collected during an outbreak. | twenty-seven giardia duodenalis cyst-positive specimens (human, animal, or drinking water) were obtained from a waterborne outbreak in a community in british columbia, western canada. parasite isolates were characterized using molecular techniques at 4 different steps of organism retrieval. none of the drinking water samples (n = 20) infected gerbils and none was successfully amplified using polymerase chain reaction (pcr). we were able to genotype 4 of 7 (human and animal) isolates by amplifica ... | 2000 | 10701587 |
| giardia and cryptosporidium in dairy calves in british columbia. | a study was undertaken to determine the prevalence of giardia infections in dairy calves and to compare giardia and cryptosporidium infections in calves of different ages. fresh fecal samples were collected from 386 male and female holstein calves (newborn to 24 wk) in 20 dairies located in the lower fraser river valley area of british columbia. giardia intestinalis, cryptosporidium parvum, and cryptosporidium muris were enumerated in each sample after concentration by sucrose gradient centrifug ... | 1997 | 9360789 |
| pulsed-field gel electrophoresis as a method of biotyping of giardia duodenalis. | pulsed-field gel electrophoresis (pfge) has allowed karyotypic characterization of protozoa at a species and a subspecies level. different methods of pfge have been applied to analysis of giardia isolates from the major morphologic groups and of isolates from the g. duodenalis group. we developed a four-day method of contour-clamped homogeneous electric field pfge that produced reliable chromosomal band separation for isolates from the g. duodenalis group. when a significant waterborne outbreak ... | 1993 | 8427382 |
| characterization of giardia duodenalis isolates from a waterborne outbreak. | isolates were retrieved from drinking water and from animal and human sources associated with a waterborne outbreak of giardiasis. this is the first report of water-source and epidemic-associated giardia isolates being adapted to in vitro propagation. outbreak-associated, non-out-break-associated, and reference isolates were characterized using isoenzyme electrophoresis and pulsed-field gel electrophoresis (pfge). all outbreak-associated and 2 other isolates were in one of eight zymodemes. the c ... | 1993 | 8421176 |
| isoenzyme analysis of human and animal isolates of giardia duodenalis from british columbia, canada. | isoenzyme patterns of 32 isolates of giardia duodenalis, obtained from 6 beavers and 11 humans from british columbia, plus 15 other isolates were evaluated using thin-layer starch-gel electrophoresis. we attempted to use 12 enzymes; 9 gave reproducible and interpretable results. the isoenzyme patterns of the isolates were classified into 12 groups with 17 (53%) of the 32 isolates confined to 1 group. the other 11 groups each comprised only 1 or 2 isolates. there was no obvious correlation betwee ... | 1989 | 2802023 |
| immunoblot patterns of giardia duodenalis isolates from different hosts and geographical locations. | eighteen isolates of giardia duodenalis from animal and human sources were studied for protein differences by polyacrylamide gel electrophoresis and for antigenic differences by immunoblot analysis. the polyacrylamide gels showed that whilst the isolates were for the most part homogeneous in their protein banding patterns, some isolates did show some differences. the immunoblot analysis yielded many bands, including prominent bands of 32 and 66 kilodaltons. five of the six isolates that showed d ... | 1990 | 2334873 |