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francisella-like endosymbiont dna and francisella tularensis virulence-related genes in brazilian ticks (acari: ixodidae).ticks are vectors of a variety of pathogens, including francisella tularensis. bacteria in the genus francisella have been identified mostly in the northern hemisphere and include tick endosymbionts. francisella has never been described in brazil, where amblyomma spp. ticks are known as the vector of many bacterial zoonotic pathogens. in the present work, we have identified bacterial dna sequences with identity to francisella genes in amblyomma dubitatum neumann dermacentor nitens (neumann), and ...200919351090
[characterization of ectoparasites on dogs in the nucleus of urban expansion of juiz de fora, minas gerais, brazil].the present work studied the characterization of ectoparasites on dogs in the nucleus of urban expansion of juiz de fora, minas gerais, brazil. the collections were conducted from july to september 2003, examining 101 dogs of no defined breed. the ectoparasites were collected through visual and tactile inspection of the animals' bodies, and were then placed in bottles with 70 degrees gl ethanol and identified under a stereomicroscope. specimens were clarified and mounted on slides for analysis u ...200819265575
[control of rhipicephalus sanguineus (latrielle, 1806) (acari: ixodidae) in the kennel of the ufmg veterinary school, belo horizonte, minas gerais, brazil].the present work was aimed at proposing a control measure for rhipicephalus sanguineus in a naturally infected kennel of 72 m(2) of the ufmg veterinary school with 25 dogs of different breeds. a sensitivity test to acaricide products was applied in a sample of r. sanguineus. out of the acaricide products tested: 12.5% amitraz (product 1); 2% alfamethrin and 60% dichlorvos association (product 2); 5% deltamethrin (product 3) and the association between 77.6% trichlorfon, 1.0% coumaphos and 1.0% c ...200818554440
sero-prevalence and risk indicators for canine ehrlichiosis in three rural areas of brazil.ehrlichia canis has a worldwide geographic distribution, occurring particularly in tropical and subtropical areas. in brazil, the main vector in urban areas is believed to be the brown dog tick rhipicephalus sanguineus, but little is known about the occurrence, transmission and other epidemiological aspects of canine ehrlichiosis in rural areas, where amblyomma ticks are found more frequently than r. sanguineus. a sero-prevalence study of canine ehrlichiosis was carried out in three distinct rur ...200717204439
canine babesiosis caused by babesia canis vogeli in rural areas of the state of minas gerais, brazil and factors associated with its seroprevalence.this epidemiological survey on canine babesiosis was carried out in three distinct rural regions (lavras, belo horizonte and nanuque) of the state of minas gerais, brazil. ticks and blood samples were collected during a dry season (lavras, n=92; belo horizonte, n=50; nanuque, n=102) and the subsequent rainy season (lavras, n=71; belo horizonte, n=28; nanuque, n=66) from dogs living on farms. plasma samples were analyzed by the indirect fluorescent antibody test for detection of anti-babesia cani ...200918723199
[drop off rhythm of rhipicephalus sanguineus (acari: ixodidae) of artificially infested dogs].the present study evaluated the drop-off rhythm of rhipicephalus sanguineus (latreille) ticks from two populations from brazil, one from monte negro, state of rondônia, and another from belo horizonte, state of minas gerais. artificial infestations with ticks were performed on dogs in the laboratory, held in a light: scotophase regimen of 12:12 h. larval drop-off rhythm was characterized by similar number of engorged larvae detaching during both periods of light and scotophase, or by a larger nu ...200819245759
population dynamics of rhipicephalus sanguineus (latrielle, 1806) in belo horizonte, minas gerais state, brazil.rhipicephalus sanguineus ticks are distributed throughout the world, especially in those areas in which dogs are in close contact with humans. r. sanguineus and fleas are regarded as the main ectoparasites infesting dogs in brazil. besides causing direct damage during the blood feeding process, this tick species can also transmit pathogens to dogs and humans. despite its importance in brazil, data regarding the seasonality of r. sanguineus are limited, especially with regard to natural infestati ...200919339119
Perceptions and attitudes among milk producers in Minas Gerais regarding cattle tick biology and control.This study evaluates milk producers' knowledge regarding cattle ticks and practices for controlling them. Ninety-three dairymen in Minas Gerais were interviewed. These producers had no information regarding acaricide efficiency tests. To analyze the information, open responses were categorized through "content analysis", and descriptive analysis consisting of extracting the profile highlighted by the highest frequencies. The association between schooling level and knowledge was tested by means o ...201121961747
Perceptions of milk producers from Divinópolis, Minas Gerais, regarding Rhipicephalus (Boophilus) microplus control.Semi-systematized interviews were conducted with 100 dairy cattle producers in the municipality of Divinópolis, Minas Gerais, with the aim of ascertaining their perceptions regarding the importance of Rhipicephalus (Boophilus) microplus and of combating it. Content analysis was performed and the frequency distribution of each of the variables was used to construct profiles of the producers interviewed. The production losses caused by ticks were perceived incompletely by the producers, who were u ...201122166383
[evaluation of ectoparasites and hemoparasites in dogs kept in apartments and houses with yards in the city of juiz de fora, minas gerais, brazil].fleas and ticks transmit various pathogens while feeding on the blood of dogs. this study sought to verify the occurrence of ectoparasitism and hemoparasitism in dogs from two urban areas in the city of juiz de fora, minas gerais, brazil. between february and august 2003, 101 dogs were studied: 50 came from apartments in the downtown region and 51 from houses with grassy yards. the ectoparasites were collected and conserved in etanol 70%. the occurrence of hemoparasites was verified by examining ...200616646996
[characterization of rickettsia spp. circulating in a silent peri-urban focus for brazilian spotted fever in caratinga, minas gerais, brazil].the present study was intended to characterize rickettsia spp. circulating in arthropod vectors in caratinga, minas gerais, brazil, by pcr and to investigate the presence of antibodies against the spotted fever rickettsiae group (sfrg) in dogs and horses. 2,610 arthropods were collected and taxonomically identified. dna samples obtained from these vectors were submitted to pcr and cycle-sequenced. ctenocephalides and amblyomma cajennense showed sequences presenting 100.0% homology with r. felis. ...200616583093
rickettsiae-infected ticks in an endemic area of spotted fever in the state of minas gerais, brazil.a study on tick-borne rickettsiosis was developed in the county of santa cruz do escalvado, state of minas gerais, brazil, where a clinical case of the disease, confirmed by necropsy, had been reported. of the 1,254 ticks collected, 1,061 belonged to the amblyomma genus, 57 to the rhipicephalus sanguineus species, 81 to boophilus microplus, and 46 to anocentor nitens. the hemolymph test associated with giménez staining showed that 18 of the 221 a. cajennense specimens, 1 of the 16 r. sanguineus, ...19979361740
strategic control of cattle ticks: milk producers' perceptions.in order to evaluate milk producers' knowledge about tests on tick sensitivity to acaricides, efficiency tests and strategic control, questionnaires were delivered to 670 producers. from these, 163 responses were received. these producers had sent ingurgitated female ticks to be subjected to tests on tick sensitivity to acaricides at embrapa between 2001 and 2005. most of the completed questionnaires came from the state of minas gerais, brazil. after descriptive analysis and correlation of varia ...201121722490
perceptions about the biology of rhipicephalus (boophilus) microplus among milk producers in divinópolis, minas gerais.one hundred semi-systematized interviews were applied with the aim of surveying the perceptions of milk producers in the municipality of divinópolis, minas gerais regarding the biology of the tick rhipicephalus (boophilus) microplus. content analysis was conducted on each of the variables surveyed and their descriptions, highlighting the higher frequencies in order to construct profiles of perceptions about each of the matters surveyed. in addition, each of the producers was categorized regardin ...201122166382
rickettsial infections of dogs, horses and ticks in juiz de fora, southeastern brazil, and isolation of rickettsia rickettsii from rhipicephalus sanguineus ticks.the present study was performed in an area endemic for brazilian spotted fever (bsf) in juiz de fora, state of minas gerais, brazil, during the years 2007 and 2008, when fatal cases of bsf (caused by rickettsia rickettsii) were reported. adult ticks (acari: ixodidae) identified as rhipicephalus sanguineus (latreille) and amblyomma cajennense (fabricius) were collected from dogs and horses, respectively, and tested by polymerase chain reaction (pcr). overall, 13.1% of the rh. sanguineus ticks and ...201020950356
association between the prevalence of infestation by rhipicephalus sanguineus and ctenocephalides felis felis and the presence of anti-leishmania antibodies: a case-control study in dogs from a brazilian endemic area.the association between the prevalence of infestation by rhipicephalus sanguineus and ctenocephalides felis felis and the presence of anti-leishmania antibodies has been evaluated in dogs located in a city of brazil endemic for canine visceral leishmaniasis. blood samples from 5556 domestic dogs domiciliated in the urban area of belo horizonte (minas gerais state) were submitted to enzyme linked immunosorbent (elisa) and indirect immunofluorescent antibody (ifat) assays, and 432 (7.8%) animals t ...201020869131
occurrence of hepatozoon sp. in dogs in the urban area originating from a municipality in southeastern brazil.the occurrence of hepatozoon sp. infection in dogs was evaluated in the urban area of uberlândia, minas gerais, brazil. the study involved 300 animals, 120 from the veterinary hospital of the federal university of uberlândia's faculty of veterinary medicine, 80 from private clinics, and 100 from the animal protective association (apa). among these animals, 7.66% presented hepatozoon sp. gamonts inside neutrophils. no statistically significant difference was found among the diagnoses based on cap ...201020810218
ticks (acari: ixodidae) on dogs from uberlândia, minas gerais, brazil.uberlândia in minas gerais state, southeastern brazil, has 622 000 inhabitants and is located in the cerrado biome, the south american savannah. the city dog population is estimated at 82 000 and identification of tick species and infestation prevalence on this host has not been determined. a major infectious disease of dogs in the city, canine ehrlichiosis, is transmitted by rhipicephalus sanguineus ticks. at the same time, autochthonous leishmaniosis has been recently described in the city and ...201020537111
ticks (acari: ixodidae) within various phytophysiognomies of a cerrado reserve in uberlândia, minas gerais, brazil.cerrado biome, the south american savannah, covers about 2 million km(2) and is very rich in endemic species but threatened by agriculture. in this report free-living tick species are presented, and their seasonal and relative distribution within the various phytophysiognomies in a small cerrado reserve in minas gerais state, brazil. overall 2,694 free-living ticks were found during a 2 years sampling period with co(2) traps and cloth dragging. of these, 73.5% were amblyomma cajennense and 0.6% ...201019693680
ticks on birds in a forest fragment of brazilian cerrado (savanna) in the municipality of uberlândia, state of minas gerais, brazil.this is a report of tick species, parasite prevalence and infestation intensity of birds in a forest fragment (18° 56' 57'' s and 48° 12' 14'' w) within the brazilian cerrado (savanna), in the municipality of uberlândia, state of minas gerais, brazil. a total of 162 birds from 26 species were captured. one adult tick, 296 larvae and 67 nymphs were found on passerine birds. of these, it was identified 31 larvae and 27 nymphs of amblyomma longirostre, 17 nymphs of a. nodosum, one a. cajennense lar ...201021184702
detection of theileria and babesia in brown brocket deer (mazama gouazoubira) and marsh deer (blastocerus dichotomus) in the state of minas gerais, brazil.intraerythrocytic protozoan species of the genera theileria and babesia are known to infect both wild and domestic animals, and both are transmitted by hard-ticks of the family ixodidae. the prevalences of hemoprotozoa and ectoparasites in 15 free-living mazama gouazoubira, two captive m. gouazoubira and four captive blastocerus dichotomus from the state of minas gerais, brazil, have been determined through the examination of blood smears and the use of nested polymerase chain reaction (npcr). t ...201021354704
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