| [occurrence of human astrovirus in são paulo city, brazil]. | human astrovirus was detected during a 13-month longitudinal study of the incidence of diarrhoea viruses among hospitalized children (less than 2 years of age) in a pediatric clinic of the city of s. paulo (university hospital). serial fecal samples (intervals of 4 days) were collected from 146 children with and without acute diarrhoea at admission and during their stay in the hospital. two (3%) of the 67 children with diarrhoea were positive at admission to the clinic by the highly sensitive as ... | 1991 | 1784973 |
| intrafamilial outbreak of astrovirus gastroenteritis in são paulo, brazil. | this report describes an intrafamilial outbreak of astrovirus-associated gastroenteritis that occurred among 5 children in a family living in the state of são paul, brazil. astrovirus was detected by direct electron microscopy (dem) and confirmed by solid phase immune electron microscopy (spiem) using astrovirus reference serum (t2-6-89). the rise in titre of astrovirus antibody in patients' sera was observed by iem using acute and convalescent-phase sera. combined use of the em techniques to de ... | 1994 | 7868830 |
| bacterial, yeast, parasitic, and viral enteropathogens in hiv-infected children from são paulo state, southeastern brazil. | we present here the frequency of enteropathogens in an hiv-infected children group and investigate their correlation with clinical and sociodemographic characteristics by collecting 100 stool samples from 55 hiv-seropositive brazilian children. all specimens were processed according to standard methods for bacterial and yeast detection. a commercially available enzyme-linked immunosorbent assay was used to detect protozoan, and to perform virus detection, molecular tests were applied. consumptio ... | 2007 | 17178297 |
| molecular characterization of astrovirus in stool samples from children in são paulo, brazil. | the purpose of this study was to characterize astrovirus in faecal samples collected from children with and without diarrhea in são paulo, brazil, grouped into two sets: epm and hu. detection and genotyping were carried out using reverse transcription nested polymerase chain reaction (rt-pcr) with specific primers directed towards the genome open reading frame 2 (orf2). results for epm set showed that 66/234 (28.2%) were positive: 28/94 (29.7%) from children with acute diarrhea, 14/45 (31.1%) wi ... | 2007 | 18209936 |