Publications

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molecular characterization of astroviruses by reverse transcriptase pcr and sequence analysis: comparison of clinical and environmental isolates from south africa.a comparative analysis was performed with 25 isolates of astroviruses (astvs) detected in sewage sources and 22 concurrently identified clinical astv isolates from the tshwane (pretoria) metropolitan area in south africa. the samples and specimens were screened for astvs by using an enzyme immunoassay and/or a reverse transcriptase pcr (rt-pcr) for the highly conserved untranslated region (3' end) of the genome. the rt-pcr results were confirmed by oligonucleotide probe dot blot hybridization. v ...200312570991
the occurrence of hepatitis a and astroviruses in selected river and dam waters in south africa.over a period of one year (june 1997-may 1998) samples of surface waters, used for domestic and recreational purposes, were collected weekly from the same sites on the klip river and vaal dam, gauteng, south africa. sensitive and specific reverse transcriptase-polymerase chain reaction-oligonucleotide probe assays were used to detect hav and hastv rna in concentrates of the water and infectious virus in cell cultures infected with the water concentrates. hav was detected in 18 (35.3%) of the riv ...200111456164
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