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methanoculleus chikugoensis sp. nov., a novel methanogenic archaeon isolated from paddy field soil in japan, and dna-dna hybridization among methanoculleus species.a strictly anaerobic, irregularly coccoid, methanogenic archaeon, strain mg62t (= jcm 10825t = dsm 13459t), was isolated from paddy field soil in chikugo, fukuoka, japan. the cells stained gram-negative, were 1.0-2.0 microm in diameter, were lysed by sds and hypotonic solutions and were flagellated. motility was not observed. the strain was able to use h2/co2, 2-propanol/co2, formate, 2-butanol/co2 and cyclopentanol/co2 as substrates for methanogenesis, but did not utilize acetate, ethanol, meth ...200111594593
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