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molecular characterisation of the metallo-beta-lactamase genes in imipenem-resistant gram-negative bacteria from a university hospital in southern taiwan.in this study, 260 non-replicate imipenem-resistant gram-negative bacteria isolated between january 2002 and december 2006 were subjected to a screening test for detection of metallo-beta-lactamase (mbl) using the etest containing imipenem and ethylene diamine tetra-acetic acid (edta). among the 260 strains, 123 (47.3%) appeared to produce mbl. of these 123 strains, 113 (91.9%) were found by polymerase chain reaction (pcr) to carry mbl genes of types blavim-2, blavim-3, blavim-11 (blavim-11a), b ...200818804966
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