| white-tailed deer as a potential reservoir of ehrlichia spp. | we determined the antibody prevalence to ehrlichia spp., in white-tailed deer (odocoileus virginianus) and the geographic distribution of seropositive animals in 84 counties in alabama, arkansas, florida, georgia, illinois, kentucky, louisiana, maryland, massachusetts, mississippi, missouri, north carolina, south carolina, tennessee, texas, virginia, and west virginia (usa). using an indirect fluorescent antibody test we detected antibodies (> or = 1:128) to this bacterium in 544 (43%) of 1269 d ... | 1994 | 8028099 |
| immunoblot analysis of the immunoglobulin g response to ehrlichia canis in dogs: an international survey. | historically, considerable variation has been reported in the type and severity of clinical and hematologic abnormalities associated with canine ehrlichiosis. because of difficulties associated with the isolation of intracellular monocytic ehrlichia species in tissue culture systems, few e. canis isolates are available for comparative microbiologic studies. to address the issue of potential e. canis antigenic diversity in different regions of the world, dog sera reactive by indirect fluorescent ... | 1997 | 9087922 |
| short report: geographic distribution of different genetic types of ehrlichia chaffeensis. | the 120-kd protein gene of ehrlichia chaffeensis was used to characterize ehrlichial dna from seven pools of adult amblyomma americanum ticks. ticks from missouri, kentucky, and north carolina contained e. chaffeensis dna of the arkansas strain genotype. ticks from north carolina also contained ehrlichiae of the sapulpa strain genotype, originally identified in oklahoma. | 1997 | 9230803 |
| serologic evidence of infection with ehrlichia spp. in wild rodents (muridae: sigmodontinae) in the united states. | rodent (muridae: sigmodontinae) blood and sera collected from 14 states were tested for seroreactivity to a cultured isolate of the human granulocytic ehrlichiosis (hge) agent by using an indirect immunofluorescence assay. of the 1,240 samples tested, 136 (11%) were found to be reactive at titers of > or = 32. rodents with hge agent-specific antibodies were found in new york (23% of 491 samples; geometric mean endpoint titer [gmt] = 441), connecticut (11% of 100 samples; gmt = 481), california ( ... | 1998 | 9508298 |
| erythema migrans-like rash illness at a camp in north carolina: a new tick-borne disease? | borrelia burgdorferi, the causative agent of lyme disease, has never been isolated from a patient thought to have acquired lyme disease in any southeastern state. | 1997 | 9531233 |
| granulocytic ehrlichiosis in dogs from north carolina and virginia. | medical records of 3 dogs from north carolina and 3 dogs from virginia with ehrlichial morulae in circulating neutrophils were studied retrospectively. two clinically distinct disease syndromes, including chronic, moderate to severe anemia (n = 3) and polyarthritis (n = 2) were associated with canine granulocytic ehrlichiosis (cge) in these dogs. one dog was clinically healthy, and abnormalities were not detected during physical examination. clinical signs were nonspecific and included fever, le ... | 1998 | 9560760 |
| minimum infection rate of ambylomma americanum (acari: ixodidae) by ehrlichia chaffeensis (rickettsiales: ehrlichieae) in southern indiana. | in 1994 and 1995, 8 cases of human monocytic ehrlichiosis were confirmed. these cases originated from southern counties where the putative tick vector ambylomma americanum (l.) is well established. to confirm the presence of ehrlichia chaffeensis in ticks in southern indiana and to determine the minimum infection rate, specimens of a. americanum were collected from 5 counties (7 sites). nucleic acid was isolated from 88 pools of ticks (430 individuals) using an optimized phenol/ctab (cetyltrimet ... | 1998 | 9775587 |
| antibodies to granulocytic ehrlichiae in white-footed and cotton mice in eastern united states. | serum samples, collected from peromyscus leucopus (white-footed mouse) or peromyscus gossypinus (cotton mouse) during 1987 through 1990 in florida, georgia, maryland, mississippi, and north carolina (usa), and in 1997 in southern connecticut were analyzed by indirect fluorescent antibody (ifa) staining methods or western blot procedures for antibodies to granulocytic ehrlichiae. of the 82 sera from white-footed mice in connecticut tested by ifa methods with either the bds or nch-1 strain of the ... | 1999 | 10231752 |
| a retrospective study of ehrlichiosis in 62 dogs from north carolina and virginia. | the purpose of this retrospective study is to report the clinical signs, clinicopathological findings, and response to therapy of 62 dogs from north carolina and virginia. ehrlichiosis was diagnosed in all of these dogs, and previous retrospective studies of ehrlichiosis in dogs were used as a basis for comparison. in this study, the clinical signs commonly associated with ehrlichiosis were observed less frequently than in earlier studies, although previously reported laboratory abnormalities we ... | 1999 | 10357108 |
| the incidence of ehrlichial and rickettsial infection in patients with unexplained fever and recent history of tick bite in central north carolina. | we examined the clinical and laboratory findings of a consecutive series of patients from central north carolina presenting with fever and a history of tick bite within the preceding 14 days. evidence of a tick-transmitted pathogen was detected in 16 of 35 patients enrolled over a 2-year period. nine patients were infected with ehrlichia chaffeensis, and 6 were infected with a spotted fever group rickettsia; 1 patient had evidence of coinfection with e. chaffeensis and a spotted fever group rick ... | 1999 | 10438390 |
| seroprevalence of ehrlichia canis, ehrlichia equi, and ehrlichia risticii in sick dogs from north carolina and virginia. | ehrlichia canis, e. equi, and e. risticii seroprevalence was determined by microimmunofluorescent antibody testing (ifa) in a sequential population of 1,845 sick dogs admitted during a 1-year period to the north carolina state university veterinary teaching hospital. a seroreactor was defined by a reciprocal ifa titer of > or =80 to e. canis, e. equi, or e. risticii antigens. of the 48 ifa seroreactors, 44 dogs were seroreactive to e. canis, 21 to e. equi, and 0 to e. risticii. seventeen dogs re ... | 2000 | 10668817 |
| prevalence of ehrlichia ewingii in amblyomma americanum in north carolina. | | 2000 | 10979750 |
| causes of uveitis in dogs: 102 cases (1989-2000). | uveitis is one of the most common ocular diseases and one of the most common causes of blindness in dogs. the purpose of this retrospective study was to correlate the signalment, history, clinical signs and ophthalmic findings of dogs with uveitis with the underlying etiology. we conducted a retrospective study of 102 dogs presented to the ncsu-vth from 1989 to 2000 with clinical signs of uveitis. medical records of dogs presented for uveitis were reviewed. dogs were included in the study only i ... | 2002 | 12071865 |
| naturally occurring ehrlichia chaffeensis infection in two prosimian primate species: ring-tailed lemurs (lemur catta) and ruffed lemurs (varecia variegata). | a naturally occurring infection of ehrlichia chaffeensis in lemurs is described. dna of ehrlichia chaffeensis was identified by polymerase chain reaction in peripheral blood from six of eight clinically ill lemurs. organisms were cultured from the blood of one lemur exhibiting clinical and hematologic abnormalities similar to those of humans infected with e. chaffeensis. | 2002 | 12498671 |
| characterization of the major antigenic protein 2 of ehrlichia canis and ehrlichia chaffeensis and its application for serodiagnosis of ehrlichiosis. | canine monocytic ehrlichiosis, caused by ehrlichia canis or ehrlichia chaffeensis, can result in clinical disease in naturally infected animals. coinfections with these agents may be common in certain areas of endemicity. currently, a species-specific method for serological diagnosis of monocytic ehrlichiosis is not available. previously, we developed two indirect enzyme-linked immunosorbent assays (elisas) using the major antigenic protein 2 (map2) of e. chaffeensis and e. canis. in this study, ... | 2003 | 12853379 |
| antinuclear antibodies can be detected in dog sera reactive to bartonella vinsonii subsp. berkhoffii, ehrlichia canis, or leishmania infantum antigens. | the presence of antinuclear antibodies (anas) is used to support a clinical diagnosis of systemic lupus erythematosus (sle) in dogs. however, clinicians must interpret the detection of anas with caution, particularly in light of increasing evidence that dogs with known bacterial and protozoal infections can have high ana titers. retrospectively, medical records were reviewed for all dogs that were concurrently tested for antinuclear antigens and bartonella vinsonii (berkhoffii), ehrlichia canis, ... | 2004 | 14765731 |
| bartonella henselae igg antibodies are prevalent in dogs from southeastern usa. | in contrast to the large body of literature regarding bartonella henselae in humans and cats, there is little information about b. henselae as an infectious agent in dogs. due to the paucity of information regarding the b. henselae serology in dogs, we performed a cross-sectional serosurvey using b. henselae antigen in order to compare the seroprevalence between sick and healthy dogs from the south-eastern usa. ninety-nine sera were collected from clinically healthy dogs. three hundred and one s ... | 2004 | 15369661 |
| comparison of results for serologic testing and a polymerase chain reaction assay to determine the prevalence of stray dogs in eastern tennessee seropositive to ehrlichia canis. | to determine the prevalence of stray dogs in eastern tennessee seropositive to ehrlichia canis and examine the correlation between results for an elisa, indirect immunofluorescent antibody (ifa) test, and polymerase chain reaction (pcr) assay. | 2004 | 15478765 |
| detection of rickettsia amblyommii in association with a tick bite rash. | in the summer of 2006, an amblyomma americanum tick was removed from a woman in central north carolina, who subsequently developed a rash at the site of tick attachment. when examined by polymerase chain reaction (pcr) for borrelia, anaplasma, ehrlichia, babesia, rickettsia, and bartonella dna, only the rickettsia primers generated an amplicon, which was identified as "r. amblyommii" by sequencing. to our knowledge, this is the first case in which r. amblyommii was temporally associated with a r ... | 2007 | 18052716 |
| natural and experimental infection of white-tailed deer (odocoileus virginianus) from the united states with an ehrlichia sp. closely related to ehrlichia ruminantium. | an ehrlichia sp. (panola mountain [pm] ehrlichia sp.) closely related to ehrlichia ruminantium was recently detected in a domestic goat experimentally infested with lone star ticks (lsts, amblyomma americanum) collected from georgia, usa. the infected goat exhibited pyrexia and mild clinical pathologic abnormalities consistent with ehrlichiosis. at least two other ehrlichia species (ehrlichia chaffeensis and ehrlichia ewingii) are maintained in nature by a cycle involving lsts as the primary vec ... | 2008 | 18436670 |
| tick-borne diseases in north carolina: is "rickettsia amblyommii" a possible cause of rickettsiosis reported as rocky mountain spotted fever? | cases of rocky mountain spotted fever (rmsf) in north carolina have escalated markedly since 2000. in 2005, we identified a county in the piedmont region with high case numbers of rmsf. we collected ticks and examined them for bacterial pathogens using molecular methods to determine if a novel tick vector or spotted fever group rickettsiae (sfgr) might be emerging. amblyomma americanum, the lone star tick, comprised 99.6% of 6,502 specimens collected in suburban landscapes. in contrast, dermacen ... | 2008 | 18447622 |
| strains of ehrlichia chaffeensis in southern indiana, kentucky, mississippi, and north carolina. | rates of infection of amblyomma americanum (l.) by ehrlichia chaffeensis were compared in 100 ticks collected from sites in each of four states: indiana, north carolina, kentucky, and mississippi. the overall infection rates were similar among sites, ranging from 1 to 4%. because pathogenic differences may exist between e. chaffeensis strains, nested polymerase chain reaction (pcr) amplification of the variable-length pcr target (vlpt), and sequencing of the amplicons were performed to different ... | 2009 | 19960699 |
| bacterial pathogens in ixodid ticks from a piedmont county in north carolina: prevalence of rickettsial organisms. | in north carolina, reported human cases of tick-borne illness, specifically rocky mountain spotted fever, have escalated over the past decade. to determine the relative abundance of vectors and to estimate the risk of acquiring a tick-borne illness in peri-residential landscapes, ticks were collected in chatham county, a typical piedmont county and, samples of the ticks were tested for infection with selected bacterial pathogens using real-time quantitative polymerase chain reaction assays. tick ... | 2010 | 20455778 |
| prevalence of selected vector-borne organisms and identification of bartonella species dna in north american river otters (lontra canadensis). | trapper-killed north american river otters (lontra canadensis) in north carolina, usa, were screened for multiple vector-borne bacteria known to be pathogenic to mammals. blood was collected from 30 carcasses in 2006, from 35 in 2007, and from one live otter in 2008. samples were screened using conventional polymerase chain reaction (pcr) tests for dna from bartonella spp., ehrlichia spp., and spotted fever group rickettsia spp. all samples were negative for rickettsia spp. twelve of 30 samples ... | 2010 | 20688703 |