| 18s rdna polymerase chain reaction and sequencing in onychomycosis diagnostics. | diagnostic approaches to onychomycosis have traditionally been based on a combination of culture and microscopy. in the present study clinical specimens from 346 patients with suspected onychomycosis were analysed by 18s polymerase chain reaction (detection) followed by sequencing and subsequent database search (identification) in parallel with routine culture on agar (detection and identification). in 49 samples trichophyton rubrum was identified by culture and sequencing. in 67 additional cult ... | 2006 | 16710579 |