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a comparison of la crosse virus isolated obtained from different ecological niches and an analysis of the structural components of california encephalitis serogroup viruses and other bunyaviruses.analyses of the oligonucleotide fingerprints of the three genome ribonucleic acid (rna) species of 11 isolates of la crosse (lac) virus, obtained from various ecological niches in the northern united states and compared to those of prototype lac virus, showed that in each place from which these isolates were obtained lac variants and varieties were present with related, but distinguishable, nucleotide sequences for their large, medium, or small rna species. the rna genomes of prototypes trivitta ...1979453439
detection of arboviral rna directly from mosquito homogenates by reverse-transcription-polymerase chain reaction.many arthropod-borne viruses (arboviruses) are important human pathogens medically. the development of an effective technique to detect the viruses by using nucleic acid amplification, such as polymerase chain reaction (pcr), improves not only clinical diagnosis but also virologic surveillance of mosquito vectors in the field. in this study, the development of an improved and simplified assay is described for detection of mosquitoes infected with eastern equine encephalitis (eee) virus, cache va ...200111337046
serologic survey of cattle in the northeastern and north central united states, virginia, alaska, and hawaii for antibodies to cache valley and antigenically related viruses (bunyamwera serogroup virus).bovine sera from northeastern states (connecticut, delaware, maine, maryland, massachusetts, new york, pennsylvania, vermont, and west virginia), north central states (indiana, illinois, iowa, kentucky, michigan, minnesota, north dakota, ohio, south dakota, and wisconsin), virginia, alaska, and hawaii were examined for the presence of neutralizing antibodies to cache valley (cv), lokern (lk), main drain (md), northway (nw), and tensaw (ts) viruses. microneutralization tests were performed using ...200212363055
isolation of bunyamwera serogroup viruses (bunyaviridae, orthobunyavirus) in new york state.during routine arbovirus surveillance from 2000 to 2004 in new york state (nys), 14,788 mosquito pools making up 36 species and nine genera were inoculated onto vero cell cultures to test for abroad spectrum of viruses. forty-six percent of viruses isolated in cell culture from species, excluding culex pipiens l. and culex restuans theobald, were identified as bunyamwera serogroup viruses. here, we report the distribution and level of bunyamwera activity in nys detected during this period. we de ...200617017240
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