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detection of rickettsia massiliae in rhipicephalus sanguineus from the eastern united states.we report the first evidence of rickettsia massiliae in the brown dog tick, rhipicephalus sanguineus, from the east coast of the united states. as part of routine pathogen surveillance, dna samples from ixodid ticks were tested for spotted fever group rickettsiae by nested pcr. a r. massiliae-positive tick was collected off a beagle mix recently rescued from north carolina. infection was confirmed by partial sequence analysis of the htra, glta, ompb, ompa, and sca4 genes, which had 100% identity ...201223199270
isolation and identification of rickettsia massiliae from rhipicephalus sanguineus ticks collected in arizona.twenty rhipicephalus sanguineus ticks collected in eastern arizona were tested by pcr assay to establish their infection rate with spotted fever group rickettsiae. with a nested pcr assay which detects a fragment of the rickettsia genus-specific 17-kda antigen gene (htra), five ticks (25%) were found to contain rickettsial dna. one rickettsial isolate was obtained from these ticks by inoculating a suspension of a triturated tick into monolayers of vero e6 monkey kidney cells and xtc-2 clawed toa ...200616885311
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