| sequence analysis of infectious bronchitis virus isolates from the 1960s in the united states. | to better understand the molecular epidemiology of infectious bronchitis virus (ibv) in the united states following the introduction of commercial ibv vaccines, we sequenced the s1 and n structural protein genes of thirteen ibv field isolates collected in the 1960s. analysis of the s1 sequence showed that seven isolates were of the massachusetts (mass) genotype, five were se17, and one was of the connecticut (conn) genotype, suggesting that these three ibvs were circulating in commercial poultry ... | 2013 | 23065112 |
| molecular characterization of avian infectious bronchitis virus strains from outbreaks in argentina (2001-2008). | twenty infectious bronchitis virus isolates were recovered from broilers and layers in different outbreaks amongst commercial poultry flocks in different geographic regions of argentina from 2001 to 2008. the viruses were isolated from the tracheas, lungs, and caecal tonsils of birds that were showing respiratory signs. further analysis based on their nucleotide and amino acid sequences in hypervariable region (hvr) 1 and the intervening sequence including hvrs 1 and 2 (hvr1/2) of the s1 gene wa ... | 2009 | 19322714 |
| evaluation of the effectiveness of two infectious bronchitis virus vaccine programs for preventing disease caused by a california ibv field isolate. | infectious bronchitis virus ca99 serotype was isolated from several broiler flocks in northern california. the virus caused late-onset respiratory disease and increased airsacculitis condemnation in affected flocks despite the use of an established infectious bronchitis virus vaccination program. an experimental study compared holland/arkansas and massachusetts/arkansas vaccination protocols to determine the efficacy of commercial infectious bronchitis virus vaccines in reducing respiratory dise ... | 2007 | 17626488 |
| strains of infectious bronchitis virus on the delmarva peninsula and in arkansas. | the constant-virus variable-serum neutralization test in chicken tracheal cultures was used to serotype infectious bronchitis virus (ibv) isolants from the delmarva peninsula and arkansas. of 106 isolants obtained from delmarva in 1971-1974, 93 were found to be the jmk strain, 5 massachusetts (mass), 5 connecticut (conn), 2 a new strain designated maryland (md) 27, and 1 a new strain designated md 31. of 26 isolants obtained from arkansas between october, 1971, and february, 1973, 17 were arkans ... | 1976 | 180960 |
| attenuated live vaccine usage affects accurate measures of virus diversity and mutation rates in avian coronavirus infectious bronchitis virus. | the full-length genomes of 11 infectious bronchitis virus (ibv) field isolates from three different types of the virus; massachusetts (mass), connecticut (conn) and california (cal) isolated over a 41, 25 and 8 year period respectively, were sequenced and analyzed to determine the mutation rates and level of polymorphisms across the genome. positive selection was not detected and mutation rates ranged from 10(-4) to 10(-6)substitutions/site/year for mass and conn ibv types where attenuated live ... | 2011 | 21539870 |
| in vivo evaluation of the pathogenicity of field isolates of infectious bronchitis virus. | the pathogenicity of 13 field isolates of infectious bronchitis virus (ibv) isolated from georgia broiler farms from 1989 to 1992 was evaluated using the ibv and escherichia coli mixed-infection model. based on the clinical signs, mortality, and lesions, the isolates were classified as high, intermediate, and low in pathogenicity. the in vivo classification was compared with the serotype classification results obtained by reverse transcriptase-polymerase chain reaction-restriction fragment lengt ... | 1994 | 7832713 |
| simultaneous detection of five major serotypes of avian coronavirus by a multiplex microsphere-based assay. | avian coronavirus (commonly known as infectious bronchitis virus [ibv]) is of major economic importance to commercial chicken producers worldwide. due to the existence of multiple serotypes and variants of the virus that do not cross-protect, it is important to diagnose circulating serotypes and choose the right vaccine type for successful protection. in an effort to improve conventional diagnostic tests, a microsphere-based assay was developed and evaluated for simultaneous detection of the mos ... | 2013 | 23847226 |
| characterization of nephropathogenic infectious bronchitis virus dmv/1639/11 recovered from delmarva broiler chickens in 2011. | a limited outbreak of nephropathogenic infectious bronchitis (nib) occurred in three delmarva (dmv) commercial broiler chicken flocks in 2011. isolates of nib virus (nibv)--dmv/1639/11, dmv/3432/11, and dmv/3902/11--were characterized by sequence analysis of the n-terminal subunit (s1) of the spike (s) gene. findings indicated that the isolates were identical to each other and to pa/9579a/10, a 2010 isolate from poultry in pennsylvania. the 2010 and 2011 isolates appear to have originated from a ... | 2013 | 23678731 |
| cloacal inoculation with the connecticut strain of avian infectious bronchitis virus: an attempt to produce nephropathogenic virus by in vivo passage using cloacal inoculation. | avian infectious bronchitis virus (ibv) strain connecticut a-5968 isolated from respiratory tissue of chickens in usa in the 1960s, is considered as representative of respiratory disease causing ibv strains. specific pathogen free chicks inoculated with the strain via the cloaca replicated the virus more rapidly in their kidneys than did chicks inoculated with the same virus intratracheally. virus passaged thirteen-times via the cloaca caused stronger nephrotropism and nephropathogenicity than t ... | 1998 | 9592724 |
| variant infectious bronchitis virus isolated from indiana chickens. | infectious bronchitis virus (ibv) associated with a catarrhal tracheitis, sudden decline in egg production, and reduced shell quality was isolated from an indiana white leghorn breeder flock. it was found to be serologically different from massachusetts, connecticut, iowa 97, iowa 609, florida, arkansas 99, jmk, holte, gray and se 17 ibv serotypes. two different massachusetts vaccine strains protected chickens from respiratory signs but not against virus infection using the isolant for challenge ... | 1976 | 181740 |
| sequence comparison of avian infectious bronchitis virus s1 glycoproteins of the florida serotype and five variant isolates from georgia and california. | the infectious bronchitis virus (ibv) spike glycoprotein s1 subunit is required to initiate infection and contains virus-neutralizing and serotype-specific epitope(s). reported are the s1 gene nucleotide and predicted amino acid sequences for the florida 18288 strain and isolates ga-92, cv-56b, cv-9437, cv-1686, and 1013. these sequences were compared with previously published gene sequences of ibv strains, and phylogenetic relationships are reported. the s1 amino acid sequence of florida 18288 ... | 1998 | 9778790 |
| genotypic and phenotypic characterization of the california 99 (cal99) variant of infectious bronchitis virus. | the california 99 (cal99) variant of infectious bronchitis virus (ibv) was first recovered in 1999 from vaccinated broiler chicken flocks in central california. the s1 hypervariable region of cal99 genome was most closely related to arkansas (ark) serotype viruses. in this study, the complete genome of cal99 was sequenced, and the structural protein genes were compared with those of commonly used ibv vaccines as well as those of isolates from naturally occurring outbreaks in different parts of t ... | 2007 | 16927130 |
| prevalence of arkansas-type infectious bronchitis virus in delmarva peninsula chickens. | the prevalence of arkansas (ark)-type infectious bronchitis virus (ibv) in delmarva peninsula broiler-type chickens was determined. the immunity of 5-to-11-week-old commercial broilers was evaluated by intraocular inoculation with ark-type dpi strain (ark dpi) challenge virus and collection of tracheal swabbings 5 days later. serum ark-type antibody titers were obtained using the virus-neutralization test. eighty-five flocks were tested from january to august 1981. nearly 60% of the flocks had s ... | 1983 | 6314974 |