Publications

use of recombinant s1 spike polypeptide to develop a tcov-specific antibody elisa.	turkey coronavirus (tcov) causes diarrhoea in young turkey poults but little is known about its prevalence in the field. to address this, a portion of the s1 region of the spike glycoprotein of tcov carrying relevant b cell epitopes (amino acid positions 54-395) was cloned and expressed in escherichia coli. this protein was purified and used to develop an indirect elisa for detection of antibodies against tcov. using experimentally derived positive and negative turkey serum samples this elisa sh ...	2009	19414227
virus shedding and serum antibody responses during experimental turkey coronavirus infections in young turkey poults.	the course of turkey coronavirus (tcov) infection in young turkey poults was examined using a field isolate (tcov-mg10) from a diarrhoeal disease outbreak on a commercial turkey farm in ontario, canada. two-day-old and 28-day-old poults were inoculated orally with tcov-mg10 to examine the effect of age on viral shedding and serum antibody responses to the virus. the presence of coronavirus particles measuring 105.8+/-21.8 nm in the cloacal contents was confirmed using transmission electron micro ...	2009	19322719